Overview
- External ontology
- mediadive.medium:1486 (source link unavailable) — GBS salts modified for Thermocrinis jamiesonii
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.3}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1486.pdf
Composition
flowchart LR
CultureMech_000953["gbs_salts_modified_for_thermocrinis_jamiesonii"]
ing0_NaH2PO4["NaH2PO4"]
CultureMech_000953 --> ing0_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing0_NaH2PO4 -.-> CHEBI_37585
ing1_NaCl["NaCl"]
CultureMech_000953 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_NH4Cl["NH4Cl"]
CultureMech_000953 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000953 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000953 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_KCl["KCl"]
CultureMech_000953 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Na2SO4["Na2SO4"]
CultureMech_000953 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_Sodium_acetate_trihydrate["Sodium acetate trihydrate"]
CultureMech_000953 --> ing7_Sodium_acetate_trihydrate
CHEBI_32138["CHEBI:32138"]
ing7_Sodium_acetate_trihydrate -.-> CHEBI_32138
ing8_Sodium_thiosulfate_pentahydrate["Sodium thiosulfate pentahydrate"]
CultureMech_000953 --> ing8_Sodium_thiosulfate_pentahydrate
CHEBI_32150["CHEBI:32150"]
ing8_Sodium_thiosulfate_pentahydrate -.-> CHEBI_32150
ing9_Sodium_bicarbonate["Sodium bicarbonate"]
CultureMech_000953 --> ing9_Sodium_bicarbonate
CHEBI_32139["CHEBI:32139"]
ing9_Sodium_bicarbonate -.-> CHEBI_32139
ing10_Na2_EDTA["Na2-EDTA"]
CultureMech_000953 --> ing10_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing10_Na2_EDTA -.-> CHEBI_64734
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000953 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000953 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000953 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_000953 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000953 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000953 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000953 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
1.38 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
3 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.107 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.015 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.123 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.15 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.3 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 8 |
Sodium acetate trihydrate
C2H3O2.3H2O.Na 136.079 g/mol
|
0.27216 G_PER_L |
CHEBI:32138
sodium acetate trihydrate
|
|
| 9 |
Sodium thiosulfate pentahydrate
5H2O.2Na.O3S2 248.186 g/mol
|
0.496372 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 10 |
Sodium bicarbonate
CHO3.Na 84.006 g/mol
|
1 MILLIMOLAR |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
3.7 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0288 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0984 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.031 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.0238 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0172 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0242 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.2.
- Prepare the medium under a nitrogen atmosphere. Dispense 5 ml into 25 ml tubes fitted with rubber stoppers.
- Autoclave at 121 °C for 15 min.
- after autoklaving, add the following from anaerobic, sterile (autoclaved) stock solutions (e.g., 100x)
- Before inoculation 2% sterile O2 or 10% sterile air is added to the headspace.
- Adjust pH to 6.0 with KOH
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- gbs_salts_modified_for_thermocrinis_jamiesonii (CultureMech:000953)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.578201Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1486 |
| 2026-01-28T19:48:15.244768Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:59.859835Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000953 |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.416633+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GBS salts modified for Thermocrinis jamiesonii" to "gbs_salts_modified_for_thermocrinis_jamiesonii" for consistent matching |
| 2026-05-20T06:01:20.856279+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.3' -> {'min': 7.2, 'max': 7.3} |
| 2026-06-05T07:41:05.995270+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:58.973828+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:36.022782+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 516667963dcbface749a6e016c7af6c0de4bbc2ebf6d25c5ed13d57e2459f058 |