gbs_salts_modified_for_thermocrinis_jamiesonii

CultureMech:000953 bacterial DEFINED LIQUID
Original name: GBS salts modified for Thermocrinis jamiesonii

Overview

External ontology
mediadive.medium:1486 (source link unavailable) — GBS salts modified for Thermocrinis jamiesonii
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.3}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1486.pdf

Composition

flowchart LR
    CultureMech_000953["gbs_salts_modified_for_thermocrinis_jamiesonii"]
    ing0_NaH2PO4["NaH2PO4"]
    CultureMech_000953 --> ing0_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing0_NaH2PO4 -.-> CHEBI_37585
    ing1_NaCl["NaCl"]
    CultureMech_000953 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000953 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000953 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000953 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KCl["KCl"]
    CultureMech_000953 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Na2SO4["Na2SO4"]
    CultureMech_000953 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_Sodium_acetate_trihydrate["Sodium acetate trihydrate"]
    CultureMech_000953 --> ing7_Sodium_acetate_trihydrate
    CHEBI_32138["CHEBI:32138"]
    ing7_Sodium_acetate_trihydrate -.-> CHEBI_32138
    ing8_Sodium_thiosulfate_pentahydrate["Sodium thiosulfate pentahydrate"]
    CultureMech_000953 --> ing8_Sodium_thiosulfate_pentahydrate
    CHEBI_32150["CHEBI:32150"]
    ing8_Sodium_thiosulfate_pentahydrate -.-> CHEBI_32150
    ing9_Sodium_bicarbonate["Sodium bicarbonate"]
    CultureMech_000953 --> ing9_Sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing9_Sodium_bicarbonate -.-> CHEBI_32139
    ing10_Na2_EDTA["Na2-EDTA"]
    CultureMech_000953 --> ing10_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing10_Na2_EDTA -.-> CHEBI_64734
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000953 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000953 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000953 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_000953 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000953 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000953 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000953 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 NaH2PO4
H2O4P.Na 119.976 g/mol
1.38 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
2 NaCl
Cl.Na 58.443 g/mol
3 G_PER_L CHEBI:26710
sodium chloride
3 NH4Cl
Cl.H4N 53.492 g/mol
0.107 G_PER_L CHEBI:31206
ammonium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.015 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.123 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 KCl
Cl.K 74.551 g/mol
0.15 G_PER_L CHEBI:32588
potassium chloride
7 Na2SO4
2Na.O4S 142.043 g/mol
0.3 G_PER_L CHEBI:32149
sodium sulfate
8 Sodium acetate trihydrate
C2H3O2.3H2O.Na 136.079 g/mol
0.27216 G_PER_L CHEBI:32138
sodium acetate trihydrate
9 Sodium thiosulfate pentahydrate
5H2O.2Na.O3S2 248.186 g/mol
0.496372 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
10 Sodium bicarbonate
CHO3.Na 84.006 g/mol
1 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
11 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
3.7 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.0288 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0984 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.031 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.0238 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.0172 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.0242 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 7.2.
  2. Prepare the medium under a nitrogen atmosphere. Dispense 5 ml into 25 ml tubes fitted with rubber stoppers.
  3. Autoclave at 121 °C for 15 min.
  4. after autoklaving, add the following from anaerobic, sterile (autoclaved) stock solutions (e.g., 100x)
  5. Before inoculation 2% sterile O2 or 10% sterile air is added to the headspace.
  6. Adjust pH to 6.0 with KOH

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.578201Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1486
2026-01-28T19:48:15.244768Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:59.859835Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000953
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.416633+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GBS salts modified for Thermocrinis jamiesonii" to "gbs_salts_modified_for_thermocrinis_jamiesonii" for consistent matching
2026-05-20T06:01:20.856279+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.3' -> {'min': 7.2, 'max': 7.3}
2026-06-05T07:41:05.995270+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:58.973828+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.