acidianus_brierleyi_medium

CultureMech:000980 archaea COMPLEX LIQUID
Original name: ACIDIANUS BRIERLEYI MEDIUM

Overview

External ontology
mediadive.medium:150 (source link unavailable) — ACIDIANUS BRIERLEYI MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 1.5, 'max': 2.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium150.pdf

Composition

flowchart LR
    CultureMech_000980["acidianus_brierleyi_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000980 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_000980 --> ing1_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000980 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_000980 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000980 --> ing4_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000980 --> ing5_Yeast_extract
    ing6_Sulfur["Sulfur"]
    CultureMech_000980 --> ing6_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing6_Sulfur -.-> CHEBI_26833

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3 G_PER_L CHEBI:62946
ammonium sulfate
2 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
5 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.02 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
6 Yeast extract 0.2 G_PER_L
7 Sulfur
S 32.067 g/mol
10 G_PER_L CHEBI:26833
sulfur atom

Preparation

  1. Dissolve ingredients except yeast extract and sulfur. Adjust pH with 6 N H2SO4 to 1.5 - 2.5. Yeast extract (10% w/v in distilled water) is autoclaved separately. For sterilization of the sulfur place it in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of the autoclaved liquid basal medium.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:10.354379Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 150
2026-03-13T06:19:00.132562Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000980
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.719132+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDIANUS BRIERLEYI MEDIUM" to "acidianus_brierleyi_medium" for consistent matching
2026-05-20T06:01:07.291243+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '1.5-2.5' -> {'min': 1.5, 'max': 2.5}
2026-06-05T07:40:47.015411+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.486770+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:28.062402+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 37d28f597183bb9d254a9ad45744b0ac7ea09cf707893739342ebeaff51409ee