acidianus_brierleyi_medium

Overview

External ontology

mediadive.medium:150 (source link unavailable) — ACIDIANUS BRIERLEYI MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 1.5, 'max': 2.5}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium150.pdf

Composition

flowchart LR
    CultureMech_000980["acidianus_brierleyi_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000980 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_000980 --> ing1_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000980 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_000980 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000980 --> ing4_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000980 --> ing5_Yeast_extract
    ing6_Sulfur["Sulfur"]
    CultureMech_000980 --> ing6_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing6_Sulfur -.-> CHEBI_26833

Ingredients (7)

Preparation

1. Dissolve ingredients except yeast extract and sulfur. Adjust pH with 6 N H2SO4 to 1.5 - 2.5. Yeast extract (10% w/v in distilled water) is autoclaved separately. For sterilization of the sulfur place it in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of the autoclaved liquid basal medium.

Target organisms

No target organisms are recorded.

Provenance