thermofilum_medium

CultureMech:000988 archaea COMPLEX LIQUID
Original name: THERMOFILUM MEDIUM

Overview

External ontology
mediadive.medium:1516 (source link unavailable) — THERMOFILUM MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 5.8, 'max': 6.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1516.pdf

Composition

flowchart LR
    CultureMech_000988["thermofilum_medium"]
    ing0_Na2S_x_H2O["Na2S x H2O"]
    CultureMech_000988 --> ing0_Na2S_x_H2O
    CHEBI_76208["CHEBI:76208"]
    ing0_Na2S_x_H2O -.-> CHEBI_76208
    ing1_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_000988 --> ing1_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing1_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing2_NiCl2_x_2_H2O["NiCl2 x 2 H2O"]
    CultureMech_000988 --> ing2_NiCl2_x_2_H2O
    CHEBI_34887["CHEBI:34887"]
    ing2_NiCl2_x_2_H2O -.-> CHEBI_34887
    ing3_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000988 --> ing3_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing3_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000988 --> ing4_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing5_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000988 --> ing5_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing5_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing6_Na2SeO4["Na2SeO4"]
    CultureMech_000988 --> ing6_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing6_Na2SeO4 -.-> CHEBI_77775
    ing7_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000988 --> ing7_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing7_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000988 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_H3BO3["H3BO3"]
    CultureMech_000988 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000988 --> ing10_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing11_Biotin["Biotin"]
    CultureMech_000988 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Folic_acid["Folic acid"]
    CultureMech_000988 --> ing12_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_Folic_acid -.-> CHEBI_27470
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000988 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000988 --> ing14_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_HCl -.-> CHEBI_49105
    ing15_Riboflavin["Riboflavin"]
    CultureMech_000988 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000988 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000988 --> ing17_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_000988 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843
    ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000988 --> ing19_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing20__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_000988 --> ing20__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing20__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    ing21_Glucose["Glucose"]
    CultureMech_000988 --> ing21_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing21_Glucose -.-> CHEBI_17234
    ing22_Yeast_extract["Yeast extract"]
    CultureMech_000988 --> ing22_Yeast_extract
    ing23_KH2PO4["KH2PO4"]
    CultureMech_000988 --> ing23_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing23_KH2PO4 -.-> CHEBI_63036
    ing24_NH4Cl["NH4Cl"]
    CultureMech_000988 --> ing24_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing24_NH4Cl -.-> CHEBI_31206
    ing25_MgCl2["MgCl2"]
    CultureMech_000988 --> ing25_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing25_MgCl2 -.-> CHEBI_6636
    ing26_CaCl2["CaCl2"]
    CultureMech_000988 --> ing26_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing26_CaCl2 -.-> CHEBI_3312
    ing27_KCl["KCl"]
    CultureMech_000988 --> ing27_KCl
    CHEBI_32588["CHEBI:32588"]
    ing27_KCl -.-> CHEBI_32588

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 Na2S x H2O
2Na.S 78.047 g/mol
0.3 G_PER_L CHEBI:76208
sodium sulfide (anhydrous)
2 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.784 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
3 NiCl2 x 2 H2O
Cl2Ni 129.599 g/mol
0.225 G_PER_L CHEBI:34887
nickel dichloride
4 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.038 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
5 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.099 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
6 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.144 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
7 Na2SeO4
2Na.O4Se 188.936 g/mol
0.095 G_PER_L CHEBI:77775
sodium selenate
8 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.033 G_PER_L CHEBI:63939
sodium tungstate dihydrate
9 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
10 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
11 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
12 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
13 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
15 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
16 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
17 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
18 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
19 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
20 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
21 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
22 Glucose
C6H12O6 180.156 g/mol
4.0 G_PER_L CHEBI:17234
glucose
 [Merged 2 duplicates: 2.0, 2.0]
23 Yeast extract 0.7 G_PER_L [Merged 2 duplicates: 0.6, 0.1]
24 KH2PO4
H2O4P.K 136.084 g/mol
0.33 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
25 NH4Cl
Cl.H4N 53.492 g/mol
0.33 G_PER_L CHEBI:31206
ammonium chloride
26 MgCl2
2Cl.Mg 95.211 g/mol
0.33 G_PER_L CHEBI:6636
magnesium dichloride
27 CaCl2
Ca.2Cl 110.984 g/mol
0.33 G_PER_L CHEBI:3312
calcium dichloride
28 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride

Preparation

  1. Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
  2. Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
  3. Strain DSM 18924 was inoculated into anaerobic tube, containing 10ml of the medium mentioned above. Glucose (2g/l) and yeast extract (0,6g/l) were added as substrate. After 3 days of incubation culture was filtered throuth 0,22nm filter. Filtered culture broth is used for the cultivation of the strain at a concentration of 1% as a source of growth factor

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.681981Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1516
2026-01-28T19:48:35.175394Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:45.712540Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:30.209969Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:00.209462Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000988
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.804650+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMOFILUM MEDIUM" to "thermofilum_medium" for consistent matching
2026-05-20T06:02:08.389470+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '5.8-6.2' -> {'min': 5.8, 'max': 6.2}
2026-06-05T07:41:50.239849+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:36.046062+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:13.043290+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:32.943518+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 2d2ab7cd0d71430050866dddaacd7df3aa01c6c18ebe3a05cc72296f441439f5