Overview
- External ontology
- mediadive.medium:1516 (source link unavailable) — THERMOFILUM MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 5.8, 'max': 6.2}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1516.pdf
Composition
flowchart LR
CultureMech_000988["thermofilum_medium"]
ing0_Na2S_x_H2O["Na2S x H2O"]
CultureMech_000988 --> ing0_Na2S_x_H2O
CHEBI_76208["CHEBI:76208"]
ing0_Na2S_x_H2O -.-> CHEBI_76208
ing1_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_000988 --> ing1_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing1_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing2_NiCl2_x_2_H2O["NiCl2 x 2 H2O"]
CultureMech_000988 --> ing2_NiCl2_x_2_H2O
CHEBI_34887["CHEBI:34887"]
ing2_NiCl2_x_2_H2O -.-> CHEBI_34887
ing3_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000988 --> ing3_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing3_CoCl2_x_6_H2O -.-> CHEBI_53503
ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000988 --> ing4_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
ing5_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000988 --> ing5_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing5_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing6_Na2SeO4["Na2SeO4"]
CultureMech_000988 --> ing6_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing6_Na2SeO4 -.-> CHEBI_77775
ing7_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000988 --> ing7_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing7_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000988 --> ing8_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing9_H3BO3["H3BO3"]
CultureMech_000988 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000988 --> ing10_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
ing11_Biotin["Biotin"]
CultureMech_000988 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_000988 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000988 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Thiamine_HCl["Thiamine HCl"]
CultureMech_000988 --> ing14_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing14_Thiamine_HCl -.-> CHEBI_49105
ing15_Riboflavin["Riboflavin"]
CultureMech_000988 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Nicotinic_acid["Nicotinic acid"]
CultureMech_000988 --> ing16_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing16_Nicotinic_acid -.-> CHEBI_15940
ing17_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_000988 --> ing17_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_Calcium_D_____pantothenate -.-> CHEBI_31345
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_000988 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000988 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_000988 --> ing20__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing20__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing21_Glucose["Glucose"]
CultureMech_000988 --> ing21_Glucose
CHEBI_17234["CHEBI:17234"]
ing21_Glucose -.-> CHEBI_17234
ing22_Yeast_extract["Yeast extract"]
CultureMech_000988 --> ing22_Yeast_extract
ing23_KH2PO4["KH2PO4"]
CultureMech_000988 --> ing23_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing23_KH2PO4 -.-> CHEBI_63036
ing24_NH4Cl["NH4Cl"]
CultureMech_000988 --> ing24_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing24_NH4Cl -.-> CHEBI_31206
ing25_MgCl2["MgCl2"]
CultureMech_000988 --> ing25_MgCl2
CHEBI_6636["CHEBI:6636"]
ing25_MgCl2 -.-> CHEBI_6636
ing26_CaCl2["CaCl2"]
CultureMech_000988 --> ing26_CaCl2
CHEBI_3312["CHEBI:3312"]
ing26_CaCl2 -.-> CHEBI_3312
ing27_KCl["KCl"]
CultureMech_000988 --> ing27_KCl
CHEBI_32588["CHEBI:32588"]
ing27_KCl -.-> CHEBI_32588
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2S x H2O
2Na.S 78.047 g/mol
|
0.3 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
|
| 2 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.784 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 3 |
NiCl2 x 2 H2O
Cl2Ni 129.599 g/mol
|
0.225 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 4 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.038 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 5 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.099 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 6 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 7 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.095 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 8 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.033 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 9 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 17 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 18 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 19 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 21 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 22 |
Glucose
C6H12O6 180.156 g/mol
|
2.0 G_PER_L |
CHEBI:17234
glucose
|
[Collapsed 2 identical duplicates: 2.0, 2.0] |
| 23 | Yeast extract | 0.7 G_PER_L | [Merged 2 duplicates: 0.6, 0.1] | |
| 24 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 25 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 26 |
MgCl2
2Cl.Mg 95.211 g/mol
|
0.33 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 27 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.33 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 28 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Preparation
- Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
- Prepare the liquid medium. Boil the medium in a water bath and cool under N2 to room temperature. Adjust to pH 5.8-6.2. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
- Strain DSM 18924 was inoculated into anaerobic tube, containing 10ml of the medium mentioned above. Glucose (2g/l) and yeast extract (0,6g/l) were added as substrate. After 3 days of incubation culture was filtered throuth 0,22nm filter. Filtered culture broth is used for the cultivation of the strain at a concentration of 1% as a source of growth factor
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.681981Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1516 |
| 2026-01-28T19:48:35.175394Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:45.712540Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:30.209969Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:00.209462Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000988 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.804650+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THERMOFILUM MEDIUM" to "thermofilum_medium" for consistent matching |
| 2026-05-20T06:02:08.389470+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5.8-6.2' -> {'min': 5.8, 'max': 6.2} |
| 2026-06-05T07:41:50.239849+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:36.046062+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:13.043290+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:32.181051+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |