Overview
- External ontology
- mediadive.medium:1520 (source link unavailable) — HALOPIGER MEDIUM (SG)
- Applications
-
- Microbial cultivation
- pH
- 8.0
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1520.pdf
Composition
flowchart LR
CultureMech_000991["halopiger_medium_sg"]
ing0_NaCl["NaCl"]
CultureMech_000991 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_000991 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_Trisodium_citrate["Trisodium citrate"]
CultureMech_000991 --> ing2_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing2_Trisodium_citrate -.-> CHEBI_53258
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000991 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Yeast_extract["Yeast extract"]
CultureMech_000991 --> ing4_Yeast_extract
ing5_Casamino_acids["Casamino acids"]
CultureMech_000991 --> ing5_Casamino_acids
ing6_Agar["Agar"]
CultureMech_000991 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
250 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
3 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 | Yeast extract | 1 G_PER_L | ||
| 6 | Casamino acids | 7.5 G_PER_L | ||
| 7 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust pH to 8.0 before autoclaving!
- If solid medium is prepared, autoclave agar and sodium chloride separately.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- halopiger_medium_sg (CultureMech:000991)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.693517Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1520 |
| 2026-01-28T19:48:27.275014Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:00.228263Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000991 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.824751+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOPIGER MEDIUM (SG)" to "halopiger_medium_sg" for consistent matching |
| 2026-06-05T07:41:08.072931+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.768604+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:04:28.884543+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e06f1647335e132c196cb5360b87b546bfde17bcd3728dc15df235a7205c42f8 |