halopiger_medium_sg

CultureMech:000991 archaea COMPLEX SOLID_AGAR
Original name: HALOPIGER MEDIUM (SG)

Overview

External ontology
mediadive.medium:1520 (source link unavailable) — HALOPIGER MEDIUM (SG)
Applications
  • Microbial cultivation
pH
8.0
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1520.pdf

Composition

flowchart LR
    CultureMech_000991["halopiger_medium_sg"]
    ing0_NaCl["NaCl"]
    CultureMech_000991 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_000991 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_Trisodium_citrate["Trisodium citrate"]
    CultureMech_000991 --> ing2_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing2_Trisodium_citrate -.-> CHEBI_53258
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000991 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_000991 --> ing4_Yeast_extract
    ing5_Casamino_acids["Casamino acids"]
    CultureMech_000991 --> ing5_Casamino_acids
    ing6_Agar["Agar"]
    CultureMech_000991 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
250 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
3 G_PER_L CHEBI:32588
potassium chloride
3 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Yeast extract 1 G_PER_L
6 Casamino acids 7.5 G_PER_L
7 Agar 20 G_PER_L CHEBI:2509
agar
 (for solid medium)

Preparation

  1. Adjust pH to 8.0 before autoclaving!
  2. If solid medium is prepared, autoclave agar and sodium chloride separately.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.693517Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1520
2026-01-28T19:48:27.275014Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:00.228263Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000991
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.824751+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOPIGER MEDIUM (SG)" to "halopiger_medium_sg" for consistent matching
2026-06-05T07:41:08.072931+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.768604+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-10-07T13:57:59.694168+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.