Overview
- External ontology
- mediadive.medium:1526d (source link unavailable) — LIMISALSIVIBRIO (L21 LS) MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1526d.pdf
Composition
flowchart LR
CultureMech_001001["limisalsivibrio_l21_ls_medium"]
ing0_NaCl["NaCl"]
CultureMech_001001 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001001 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KCl["KCl"]
CultureMech_001001 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Na2SO4["Na2SO4"]
CultureMech_001001 --> ing3_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing3_Na2SO4 -.-> CHEBI_32149
ing4_NH4Cl["NH4Cl"]
CultureMech_001001 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001001 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_K2HPO4["K2HPO4"]
CultureMech_001001 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001001 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_Na2CO3["Na2CO3"]
CultureMech_001001 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Na_acetate["Na-acetate"]
CultureMech_001001 --> ing9_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Na_acetate -.-> CHEBI_32954
ing10_Na2_fumarate["Na2-fumarate"]
CultureMech_001001 --> ing10_Na2_fumarate
CHEBI_115156["CHEBI:115156"]
ing10_Na2_fumarate -.-> CHEBI_115156
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001001 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001001 --> ing12_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001001 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001001 --> ing14_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing14_MnSO4_x_H2O -.-> CHEBI_86364
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001001 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001001 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001001 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001001 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001001 --> ing19_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing20_H3BO3["H3BO3"]
CultureMech_001001 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001001 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001001 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001001 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001001 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing25_Vitamin_B12["Vitamin B12"]
CultureMech_001001 --> ing25_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing25_Vitamin_B12 -.-> CHEBI_176843
ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001001 --> ing26_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
ing27_D_____biotin["D-(+)-biotin"]
CultureMech_001001 --> ing27_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing27_D_____biotin -.-> CHEBI_15956
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_001001 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001001 --> ing29_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_pantothenate -.-> CHEBI_31345
CultureMech_001001 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
60.3472 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 59.3472, 1.0] |
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.93472 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
1.48368 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.98912 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.98912 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.495648 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.395648, 0.1] |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.395648 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049456 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.98912 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
1.58259 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 11 |
Na2-fumarate
C4H2O4.2Na 160.036 g/mol
|
6.33037 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49456 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 15 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 26 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 27 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 28 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 32 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- Dissolve ingredients except carbonate, acetate, fumarate, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate, fumarate, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (fumarate and vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- limisalsivibrio_l21_ls_medium (CultureMech:001001)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.733184Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1526d |
| 2026-03-13T03:09:01.357926Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:00.356933Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001001 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.963918+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LIMISALSIVIBRIO (L21 LS) MEDIUM" to "limisalsivibrio_l21_ls_medium" for consistent matching |
| 2026-05-20T06:01:24.378173+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0} |
| 2026-06-05T07:41:11.169466+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.545772+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:40.268741+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 52b95f7f4c1e50b40c973627361f76f4f5502bd08cb223203750633dd7163aec |