limisalsivibrio_l21_ls_medium

CultureMech:001001 bacterial DEFINED LIQUID
Original name: LIMISALSIVIBRIO (L21 LS) MEDIUM

Overview

External ontology
mediadive.medium:1526d (source link unavailable) — LIMISALSIVIBRIO (L21 LS) MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1526d.pdf

Composition

flowchart LR
    CultureMech_001001["limisalsivibrio_l21_ls_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001001 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001001 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KCl["KCl"]
    CultureMech_001001 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_Na2SO4["Na2SO4"]
    CultureMech_001001 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_NH4Cl["NH4Cl"]
    CultureMech_001001 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001001 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_K2HPO4["K2HPO4"]
    CultureMech_001001 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001001 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_Na2CO3["Na2CO3"]
    CultureMech_001001 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_Na_acetate["Na-acetate"]
    CultureMech_001001 --> ing9_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing9_Na_acetate -.-> CHEBI_32954
    ing10_Na2_fumarate["Na2-fumarate"]
    CultureMech_001001 --> ing10_Na2_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing10_Na2_fumarate -.-> CHEBI_115156
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001001 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001001 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001001 --> ing13_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing14_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001001 --> ing14_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing14_MnSO4_x_H2O -.-> CHEBI_86364
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001001 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001001 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001001 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001001 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001001 --> ing19_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing20_H3BO3["H3BO3"]
    CultureMech_001001 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001001 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001001 --> ing22_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001001 --> ing23_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001001 --> ing24_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001001 --> ing25_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing26_D_____biotin["D-(+)-biotin"]
    CultureMech_001001 --> ing26_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing26_D_____biotin -.-> CHEBI_15956
    ing27_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001001 --> ing27_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
60.3472 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 59.3472, 1.0]
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.93472 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KCl
Cl.K 74.551 g/mol
1.48368 G_PER_L CHEBI:32588
potassium chloride
4 Na2SO4
2Na.O4S 142.043 g/mol
0.98912 G_PER_L CHEBI:32149
sodium sulfate
5 NH4Cl
Cl.H4N 53.492 g/mol
0.98912 G_PER_L CHEBI:31206
ammonium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.495648 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.395648, 0.1]
7 K2HPO4
HO4P.2K 174.174 g/mol
0.395648 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.00049456 G_PER_L CHEBI:8806
Resazurin
9 Na2CO3
CO3.2Na 105.988 g/mol
0.98912 G_PER_L CHEBI:29377
sodium carbonate
10 Na-acetate
C2H3O2.Na 82.034 g/mol
1.58259 G_PER_L CHEBI:32954
sodium acetate
11 Na2-fumarate
C4H2O4.2Na 160.036 g/mol
6.33037 G_PER_L CHEBI:115156
disodium fumarate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.49456 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
15 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
17 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
24 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
25 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
26 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
27 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
28 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Preparation

  1. Dissolve ingredients except carbonate, acetate, fumarate, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate, fumarate, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (fumarate and vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary.
  2. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Solutions

Seven vitamins solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 0.1 G_PER_L
Nicotinic acid 0.2 G_PER_L
Pyridoxine hydrochloride 0.3 G_PER_L
Thiamine-HCl x 2 H2O 0.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.733184Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1526d
2026-03-13T03:09:01.357926Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:00.356933Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001001
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.963918+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LIMISALSIVIBRIO (L21 LS) MEDIUM" to "limisalsivibrio_l21_ls_medium" for consistent matching
2026-05-20T06:01:24.378173+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:41:11.169466+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.545772+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.437756+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> 'Seven vitamins solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).