Overview
- External ontology
- mediadive.medium:1568 (source link unavailable) — MEDIUM FOR METHYLOCALDUM (RS11D-PR)
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1568.pdf
Composition
flowchart LR
CultureMech_001044["medium_for_methylocaldum_rs11d_pr"]
ing0_NaNO3["NaNO3"]
CultureMech_001044 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001044 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Na2HPO4["Na2HPO4"]
CultureMech_001044 --> ing2_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing2_Na2HPO4 -.-> CHEBI_34683
ing3_KH2PO4["KH2PO4"]
CultureMech_001044 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001044 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001044 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001044 --> ing6_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001044 --> ing7_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
ing8_MnSO4_x_2_H2O["MnSO4 x 2 H2O"]
CultureMech_001044 --> ing8_MnSO4_x_2_H2O
CHEBI_86356["CHEBI:86356"]
ing8_MnSO4_x_2_H2O -.-> CHEBI_86356
ing9_Na2MoO4["Na2MoO4"]
CultureMech_001044 --> ing9_Na2MoO4
CHEBI_75215["CHEBI:75215"]
ing9_Na2MoO4 -.-> CHEBI_75215
ing10_H3BO3["H3BO3"]
CultureMech_001044 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001044 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.22 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.03 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.002 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.44 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 8 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 9 |
MnSO4 x 2 H2O
2H2O.Mn.O4S 187.031 g/mol
|
0.17 G_PER_L |
CHEBI:86356
manganese(II) sulfate dihydrate
|
|
| 10 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
0.06 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.08 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- Medium was adjusted pH 7.0 with NaOH
- Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 20% methane to the gas phase and autoclave at 121°C for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_for_methylocaldum_rs11d_pr (CultureMech:001044)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.866561Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1568 |
| 2026-03-13T06:19:00.711234Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001044 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.347147+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM FOR METHYLOCALDUM (RS11D-PR)" to "medium_for_methylocaldum_rs11d_pr" for consistent matching |
| 2026-06-05T07:41:29.188308+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.091316+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:43.179767+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1728163491f51776d7f04813e5b213bbf5c86937750094f301ced4e0e33302b9 |