medium_for_methylocaldum_rs11d_pr

CultureMech:001044 bacterial DEFINED LIQUID
Original name: MEDIUM FOR METHYLOCALDUM (RS11D-PR)

Overview

External ontology
mediadive.medium:1568 (source link unavailable) — MEDIUM FOR METHYLOCALDUM (RS11D-PR)
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1568.pdf

Composition

flowchart LR
    CultureMech_001044["medium_for_methylocaldum_rs11d_pr"]
    ing0_NaNO3["NaNO3"]
    CultureMech_001044 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001044 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_Na2HPO4["Na2HPO4"]
    CultureMech_001044 --> ing2_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4 -.-> CHEBI_34683
    ing3_KH2PO4["KH2PO4"]
    CultureMech_001044 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001044 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001044 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001044 --> ing6_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001044 --> ing7_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing8_MnSO4_x_2_H2O["MnSO4 x 2 H2O"]
    CultureMech_001044 --> ing8_MnSO4_x_2_H2O
    CHEBI_86356["CHEBI:86356"]
    ing8_MnSO4_x_2_H2O -.-> CHEBI_86356
    ing9_Na2MoO4["Na2MoO4"]
    CultureMech_001044 --> ing9_Na2MoO4
    CHEBI_75215["CHEBI:75215"]
    ing9_Na2MoO4 -.-> CHEBI_75215
    ing10_H3BO3["H3BO3"]
    CultureMech_001044 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001044 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 NaNO3
NO3.Na 84.994 g/mol
1 G_PER_L CHEBI:63005
sodium nitrate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 Na2HPO4
HO4P.2Na 141.958 g/mol
0.5 G_PER_L CHEBI:34683
disodium hydrogenphosphate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.22 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.03 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.002 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
7 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.44 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
8 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
9 MnSO4 x 2 H2O
2H2O.Mn.O4S 187.031 g/mol
0.17 G_PER_L CHEBI:86356
manganese(II) sulfate dihydrate
10 Na2MoO4
MoO4.2Na 205.916 g/mol
0.06 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
11 H3BO3
H3BO3 61.833 g/mol
0.1 G_PER_L CHEBI:33118
boric acid
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.08 G_PER_L CHEBI:53503
cobalt chloride hexahydrate

Preparation

  1. Medium was adjusted pH 7.0 with NaOH
  2. Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 20% methane to the gas phase and autoclave at 121°C for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.866561Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1568
2026-03-13T06:19:00.711234Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001044
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.347147+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR METHYLOCALDUM (RS11D-PR)" to "medium_for_methylocaldum_rs11d_pr" for consistent matching
2026-06-05T07:41:29.188308+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.091316+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.