Overview
- External ontology
- mediadive.medium:1585 (source link unavailable) — SULFURIFERULA MULTIVORANS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.5, 'max': 7.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1585.pdf
Composition
flowchart LR
CultureMech_001060["sulfuriferula_multivorans_medium"]
ing0_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_001060 --> ing0_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing0_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing1_KH2PO4["KH2PO4"]
CultureMech_001060 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001060 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_001060 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001060 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_001060 --> ing5_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
ing6_MnSO4_x_5_H2O["MnSO4 x 5 H2O"]
CultureMech_001060 --> ing6_MnSO4_x_5_H2O
CHEBI_131524["CHEBI:131524"]
ing6_MnSO4_x_5_H2O -.-> CHEBI_131524
ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001060 --> ing7_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
3 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.8 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.04 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.03 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 7 |
MnSO4 x 5 H2O
5H2O.Mn.O4S 241.076 g/mol
|
0.03 G_PER_L |
CHEBI:131524
manganese(II) sulfate pentahydrate
|
|
| 8 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
10 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Preparation
- Dissolve ingredients except thiosulfate and adjust pH of medium to 6.5 -7.0. Dispense medium under air atmosphere into serum vials suitable for anaerobic cultivation (e.g., 20 ml medium in 100 ml bottles) and close with butyl rubber septa. After autoclaving complete medium with thiosulfate added from a stock solution sterilized by filtration and add 3% (v/v) sterile CO2 to the gas phase.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sulfuriferula_multivorans_medium (CultureMech:001060)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.912255Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1585 |
| 2026-03-13T06:19:00.848634Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001060 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.498160+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SULFURIFERULA MULTIVORANS MEDIUM" to "sulfuriferula_multivorans_medium" for consistent matching |
| 2026-05-20T06:02:06.560504+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.5-7' -> {'min': 6.5, 'max': 7.0} |
| 2026-06-05T07:41:47.657363+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:12.105191+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.925936+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8b148f68fc355ac61121b6b14205511a1effb42fddab60315cc564c169a939d8 |