sulfuriferula_multivorans_medium

CultureMech:001060 bacterial DEFINED LIQUID
Original name: SULFURIFERULA MULTIVORANS MEDIUM

Overview

External ontology
mediadive.medium:1585 (source link unavailable) — SULFURIFERULA MULTIVORANS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.5, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1585.pdf

Composition

flowchart LR
    CultureMech_001060["sulfuriferula_multivorans_medium"]
    ing0_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_001060 --> ing0_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing0_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001060 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001060 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001060 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001060 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001060 --> ing5_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing6_MnSO4_x_5_H2O["MnSO4 x 5 H2O"]
    CultureMech_001060 --> ing6_MnSO4_x_5_H2O
    CHEBI_131524["CHEBI:131524"]
    ing6_MnSO4_x_5_H2O -.-> CHEBI_131524
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001060 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
3 G_PER_L CHEBI:34683
disodium hydrogenphosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.8 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.1 G_PER_L CHEBI:62946
ammonium sulfate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.04 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.03 G_PER_L CHEBI:86254
iron trichloride hexahydrate
7 MnSO4 x 5 H2O
5H2O.Mn.O4S 241.076 g/mol
0.03 G_PER_L CHEBI:131524
manganese(II) sulfate pentahydrate
8 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
10 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate

Preparation

  1. Dissolve ingredients except thiosulfate and adjust pH of medium to 6.5 -7.0. Dispense medium under air atmosphere into serum vials suitable for anaerobic cultivation (e.g., 20 ml medium in 100 ml bottles) and close with butyl rubber septa. After autoclaving complete medium with thiosulfate added from a stock solution sterilized by filtration and add 3% (v/v) sterile CO2 to the gas phase.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.912255Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1585
2026-03-13T06:19:00.848634Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001060
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.498160+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SULFURIFERULA MULTIVORANS MEDIUM" to "sulfuriferula_multivorans_medium" for consistent matching
2026-05-20T06:02:06.560504+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.5-7' -> {'min': 6.5, 'max': 7.0}
2026-06-05T07:41:47.657363+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:12.105191+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:57:59.694168+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.