Overview
- External ontology
- mediadive.medium:1586 (source link unavailable) — ISP2 MODIFIED MEDIUM FOR METHYLIBIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1586.pdf
Composition
flowchart LR
CultureMech_001062["isp2_modified_medium_for_methylibium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001062 --> ing0_Yeast_extract
ing1_Malt_extract["Malt extract"]
CultureMech_001062 --> ing1_Malt_extract
ing2_Dextrose["Dextrose"]
CultureMech_001062 --> ing2_Dextrose
CHEBI_17634["CHEBI:17634"]
ing2_Dextrose -.-> CHEBI_17634
ing3_Agar["Agar"]
CultureMech_001062 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_HCl["HCl"]
CultureMech_001062 --> ing4_HCl
CHEBI_17883["CHEBI:17883"]
ing4_HCl -.-> CHEBI_17883
ing5_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001062 --> ing5_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing5_FeCl2_x_4_H2O -.-> CHEBI_86249
ing6_ZnCl2["ZnCl2"]
CultureMech_001062 --> ing6_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing6_ZnCl2 -.-> CHEBI_49976
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001062 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
ing8_H3BO3["H3BO3"]
CultureMech_001062 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001062 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001062 --> ing10_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001062 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001062 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_Vitamin_B12["Vitamin B12"]
CultureMech_001062 --> ing13_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing13_Vitamin_B12 -.-> CHEBI_176843
ing14_Biotin["Biotin"]
CultureMech_001062 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001062 --> ing15_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing15_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing16_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001062 --> ing16_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing16_Calcium_pantothenate -.-> CHEBI_31345
ing17_Folic_acid["Folic acid"]
CultureMech_001062 --> ing17_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing17_Folic_acid -.-> CHEBI_27470
ing18_Riboflavin["Riboflavin"]
CultureMech_001062 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinamide["Nicotinamide"]
CultureMech_001062 --> ing19_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing19_Nicotinamide -.-> CHEBI_17154
ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001062 --> ing20_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001062 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 4 G_PER_L | ||
| 2 | Malt extract | 10 G_PER_L | ||
| 3 |
Dextrose
C6H12O6 180.156 g/mol
|
4 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 4 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 5 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 6 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 7 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 | Vitamin B12 | 0.0002 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.004 G_PER_L |
CHEBI:15956
biotin
|
|
| 16 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.01 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 17 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.01 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 18 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.001 G_PER_L |
CHEBI:27470
folic acid
|
|
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 20 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.01 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 21 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Preparation
- pH 7.2
- Sterilize 20 min/121°C. After cooling to 60°C add to the medium:
- Filter-sterilized.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- isp2_modified_medium_for_methylibium (CultureMech:001062)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.917866Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1586 |
| 2026-03-13T06:19:00.866649Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001062 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.518356+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ISP2 MODIFIED MEDIUM FOR METHYLIBIUM" to "isp2_modified_medium_for_methylibium" for consistent matching |
| 2026-06-05T07:41:09.658495+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.142517+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:38.798245+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1b8a9bc70138085b24837d96a44eedd964cd12d2a5a8708b2c392e310a906d60 |