isp2_modified_medium_for_methylibium

CultureMech:001062 bacterial COMPLEX SOLID_AGAR
Original name: ISP2 MODIFIED MEDIUM FOR METHYLIBIUM

Overview

External ontology
mediadive.medium:1586 (source link unavailable) — ISP2 MODIFIED MEDIUM FOR METHYLIBIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1586.pdf

Composition

flowchart LR
    CultureMech_001062["isp2_modified_medium_for_methylibium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001062 --> ing0_Yeast_extract
    ing1_Malt_extract["Malt extract"]
    CultureMech_001062 --> ing1_Malt_extract
    ing2_Dextrose["Dextrose"]
    CultureMech_001062 --> ing2_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing2_Dextrose -.-> CHEBI_17634
    ing3_Agar["Agar"]
    CultureMech_001062 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_HCl["HCl"]
    CultureMech_001062 --> ing4_HCl
    CHEBI_17883["CHEBI:17883"]
    ing4_HCl -.-> CHEBI_17883
    ing5_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001062 --> ing5_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing5_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing6_ZnCl2["ZnCl2"]
    CultureMech_001062 --> ing6_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing6_ZnCl2 -.-> CHEBI_49976
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001062 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_H3BO3["H3BO3"]
    CultureMech_001062 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001062 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001062 --> ing10_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001062 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001062 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_Vitamin_B12["Vitamin B12"]
    CultureMech_001062 --> ing13_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing13_Vitamin_B12 -.-> CHEBI_176843
    ing14_Biotin["Biotin"]
    CultureMech_001062 --> ing14_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_Biotin -.-> CHEBI_15956
    ing15_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001062 --> ing15_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing15_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing16_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001062 --> ing16_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_pantothenate -.-> CHEBI_31345
    ing17_Folic_acid["Folic acid"]
    CultureMech_001062 --> ing17_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing17_Folic_acid -.-> CHEBI_27470
    ing18_Riboflavin["Riboflavin"]
    CultureMech_001062 --> ing18_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing18_Riboflavin -.-> CHEBI_17015
    ing19_Nicotinamide["Nicotinamide"]
    CultureMech_001062 --> ing19_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing19_Nicotinamide -.-> CHEBI_17154
    ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001062 --> ing20_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001062 --> ing21_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Yeast extract 4 G_PER_L
2 Malt extract 10 G_PER_L
3 Dextrose
C6H12O6 180.156 g/mol
4 G_PER_L CHEBI:17634
D-glucose
4 Agar 20 G_PER_L CHEBI:2509
agar
5 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
6 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
7 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
9 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
10 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
11 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
12 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
13 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
14 Vitamin B12 0.0002 G_PER_L CHEBI:176843
vitamin B12
15 Biotin
C10H16N2O3S 244.316 g/mol
0.004 G_PER_L CHEBI:15956
biotin
16 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
17 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
18 Folic acid
C19H19N7O6 441.404 g/mol
0.001 G_PER_L CHEBI:27470
folic acid
19 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
20 Nicotinamide
C6H6N2O 122.127 g/mol
0.01 G_PER_L CHEBI:17154
nicotinamide
21 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
22 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride

Preparation

  1. pH 7.2
  2. Sterilize 20 min/121°C. After cooling to 60°C add to the medium:
  3. Filter-sterilized.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.917866Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1586
2026-03-13T06:19:00.866649Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001062
2026-03-17T06:30:20.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.518356+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ISP2 MODIFIED MEDIUM FOR METHYLIBIUM" to "isp2_modified_medium_for_methylibium" for consistent matching
2026-06-05T07:41:09.658495+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.142517+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.