Overview
- External ontology
- mediadive.medium:1646 (source link unavailable) — GEMMATIMONAS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1646.pdf
Composition
flowchart LR
CultureMech_001129["gemmatimonas_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001129 --> ing0_Yeast_extract
ing1_Peptone["Peptone"]
CultureMech_001129 --> ing1_Peptone
ing2_Glucose["Glucose"]
CultureMech_001129 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_HEPES["HEPES"]
CultureMech_001129 --> ing3_HEPES
CHEBI_46756["CHEBI:46756"]
ing3_HEPES -.-> CHEBI_46756
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001129 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_001129 --> ing5_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing5_CaSO4_x_2_H2O -.-> CHEBI_32583
ing6_Agar["Agar"]
CultureMech_001129 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Vitamin_B12["Vitamin B12"]
CultureMech_001129 --> ing7_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing7_Vitamin_B12 -.-> CHEBI_176843
ing8_Pantothenic_acid["Pantothenic acid"]
CultureMech_001129 --> ing8_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing8_Pantothenic_acid -.-> CHEBI_7916
ing9_Riboflavin["Riboflavin"]
CultureMech_001129 --> ing9_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing9_Riboflavin -.-> CHEBI_17015
ing10_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_001129 --> ing10_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing10_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing11_Biotin["Biotin"]
CultureMech_001129 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_001129 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Nicotinic_acid["Nicotinic acid"]
CultureMech_001129 --> ing13_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing13_Nicotinic_acid -.-> CHEBI_15940
ing14_Nicotine_amide["Nicotine amide"]
CultureMech_001129 --> ing14_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing14_Nicotine_amide -.-> CHEBI_17154
ing15_alpha_lipoic_acid["alpha-lipoic acid"]
CultureMech_001129 --> ing15_alpha_lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing15_alpha_lipoic_acid -.-> CHEBI_16494
ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001129 --> ing16_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
ing17_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001129 --> ing17_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing17_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.25 G_PER_L | ||
| 2 | Peptone | 0.5 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.1 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | HEPES | 1.95 G_PER_L |
CHEBI:46756
HEPES
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.37 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.43 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 7 | Agar | 14 G_PER_L |
CHEBI:2509
agar
|
|
| 8 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 9 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 10 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 11 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.01 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 15 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 16 |
alpha-lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 17 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 18 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.05 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- Adjust pH to 7.0 prior to the addition of agar.
- After autoclaving and cooling to about 55°C, add 5 ml of vitamin solution.
- Stir for some hours, filter sterilize the solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- gemmatimonas_medium (CultureMech:001129)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.109726Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1646 |
| 2026-03-13T06:19:01.444876Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001129 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.162869+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GEMMATIMONAS MEDIUM" to "gemmatimonas_medium" for consistent matching |
| 2026-06-05T07:41:06.032869+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:36.043817+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2ded48fcff98245a44cc4c0eef1e476a40f4054573468200f895c5d13890e74c |