gemmatimonas_medium

CultureMech:001129 bacterial COMPLEX SOLID_AGAR
Original name: GEMMATIMONAS MEDIUM

Overview

External ontology
mediadive.medium:1646 (source link unavailable) — GEMMATIMONAS MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1646.pdf

Composition

flowchart LR
    CultureMech_001129["gemmatimonas_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001129 --> ing0_Yeast_extract
    ing1_Peptone["Peptone"]
    CultureMech_001129 --> ing1_Peptone
    ing2_Glucose["Glucose"]
    CultureMech_001129 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_HEPES["HEPES"]
    CultureMech_001129 --> ing3_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing3_HEPES -.-> CHEBI_46756
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001129 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_001129 --> ing5_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing5_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing6_Agar["Agar"]
    CultureMech_001129 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Vitamin_B12["Vitamin B12"]
    CultureMech_001129 --> ing7_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing7_Vitamin_B12 -.-> CHEBI_176843
    ing8_Pantothenic_acid["Pantothenic acid"]
    CultureMech_001129 --> ing8_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing8_Pantothenic_acid -.-> CHEBI_7916
    ing9_Riboflavin["Riboflavin"]
    CultureMech_001129 --> ing9_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing9_Riboflavin -.-> CHEBI_17015
    ing10_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_001129 --> ing10_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing10_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing11_Biotin["Biotin"]
    CultureMech_001129 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Folic_acid["Folic acid"]
    CultureMech_001129 --> ing12_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_Folic_acid -.-> CHEBI_27470
    ing13_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001129 --> ing13_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing13_Nicotinic_acid -.-> CHEBI_15940
    ing14_Nicotine_amide["Nicotine amide"]
    CultureMech_001129 --> ing14_Nicotine_amide
    CHEBI_17154["CHEBI:17154"]
    ing14_Nicotine_amide -.-> CHEBI_17154
    ing15_alpha_lipoic_acid["alpha-lipoic acid"]
    CultureMech_001129 --> ing15_alpha_lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing15_alpha_lipoic_acid -.-> CHEBI_16494
    ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001129 --> ing16_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing17_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001129 --> ing17_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing17_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.25 G_PER_L
2 Peptone 0.5 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
0.1 G_PER_L CHEBI:17234
glucose
4 HEPES 1.95 G_PER_L CHEBI:46756
HEPES
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.37 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
0.43 G_PER_L CHEBI:32583
calcium sulfate dihydrate
7 Agar 14 G_PER_L CHEBI:2509
agar
8 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
9 Pantothenic acid
C9H17NO5 219.237 g/mol
0.05 G_PER_L CHEBI:7916
pantothenic acid
10 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
11 Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
0.01 G_PER_L CHEBI:131531
pyridoxamine hydrochloride
12 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
13 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
14 Nicotinic acid
C6H5NO2 123.111 g/mol
0.025 G_PER_L CHEBI:15940
nicotinic acid
15 Nicotine amide
C6H6N2O 122.127 g/mol
0.025 G_PER_L CHEBI:17154
nicotinamide
16 alpha-lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
17 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
18 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.05 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate

Preparation

  1. Adjust pH to 7.0 prior to the addition of agar.
  2. After autoclaving and cooling to about 55°C, add 5 ml of vitamin solution.
  3. Stir for some hours, filter sterilize the solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.109726Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1646
2026-03-13T06:19:01.444876Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001129
2026-03-17T06:30:02.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.162869+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GEMMATIMONAS MEDIUM" to "gemmatimonas_medium" for consistent matching
2026-06-05T07:41:06.032869+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.