nitrospira_inopinata_medium

Overview

External ontology

mediadive.medium:1690 (source link unavailable) — NITROSPIRA INOPINATA MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.6, 'max': 8.0}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1690.pdf

Composition

flowchart LR
    CultureMech_001172["nitrospira_inopinata_medium"]
    ing0_CaCO3["CaCO3"]
    CultureMech_001172 --> ing0_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing0_CaCO3 -.-> CHEBI_3311
    ing1_H3BO3["H3BO3"]
    CultureMech_001172 --> ing1_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing1_H3BO3 -.-> CHEBI_33118
    ing2_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001172 --> ing2_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing2_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing3_HCl["HCl"]
    CultureMech_001172 --> ing3_HCl
    CHEBI_17883["CHEBI:17883"]
    ing3_HCl -.-> CHEBI_17883
    ing4_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001172 --> ing4_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing4_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing5_ZnCl2["ZnCl2"]
    CultureMech_001172 --> ing5_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing5_ZnCl2 -.-> CHEBI_49976
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001172 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001172 --> ing7_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001172 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001172 --> ing9_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing10_NaOH["NaOH"]
    CultureMech_001172 --> ing10_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing10_NaOH -.-> CHEBI_32145
    ing11_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001172 --> ing11_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing11_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing12_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001172 --> ing12_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing12_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing13_Ammonium_chloride["Ammonium chloride"]
    CultureMech_001172 --> ing13_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing13_Ammonium_chloride -.-> CHEBI_31206
    ing14_KH2PO4["KH2PO4"]
    CultureMech_001172 --> ing14_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing14_KH2PO4 -.-> CHEBI_63036
    ing15_KCl["KCl"]
    CultureMech_001172 --> ing15_KCl
    CHEBI_32588["CHEBI:32588"]
    ing15_KCl -.-> CHEBI_32588
    ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001172 --> ing16_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing17_NaCl["NaCl"]
    CultureMech_001172 --> ing17_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing17_NaCl -.-> CHEBI_26710

Ingredients (18)

Preparation

1. Dilute stock solution with MilliQ water (1+19 v/v), add 4 g CaCO3 per litre.
2. Autoclave at 120 °C for 21 min.
3. Cool down to room temperature.
4. After autoclaving add sterile solutions:
5. Check pH 7.6-8.0
6. Complete medium can be stored at 4°C.
7. Incubate at 42°C without shaking. Activity and growth can be followed by measuring the depletion of ammonium or the production of nitrite and/or nitrate. Higher ammonium concentrations are inhibitive. For that reason our recommendation is follow nitrite content. As soon as nitrite has been produced and is depleting again (to form nitrate) another dose of ammonium can be added. Incubation time may be up to several weeks. We checked the nitrite content (absence/presence) every 5-7 days and added ammonium chloride about every 2-3 weeks. Don't expose cultures to intense light.
8. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
9. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MQ water.
10. Autoclave at 120 °C for 21 min.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• nitrospira_inopinata_medium (CultureMech:001172)