nitrospira_inopinata_medium

CultureMech:001172 bacterial DEFINED LIQUID
Original name: NITROSPIRA INOPINATA MEDIUM

Overview

External ontology
mediadive.medium:1690 (source link unavailable) — NITROSPIRA INOPINATA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.6, 'max': 8.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1690.pdf

Composition

flowchart LR
    CultureMech_001172["nitrospira_inopinata_medium"]
    ing0_CaCO3["CaCO3"]
    CultureMech_001172 --> ing0_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing0_CaCO3 -.-> CHEBI_3311
    ing1_H3BO3["H3BO3"]
    CultureMech_001172 --> ing1_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing1_H3BO3 -.-> CHEBI_33118
    ing2_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001172 --> ing2_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing2_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing3_HCl["HCl"]
    CultureMech_001172 --> ing3_HCl
    CHEBI_17883["CHEBI:17883"]
    ing3_HCl -.-> CHEBI_17883
    ing4_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001172 --> ing4_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing4_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing5_ZnCl2["ZnCl2"]
    CultureMech_001172 --> ing5_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing5_ZnCl2 -.-> CHEBI_49976
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001172 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001172 --> ing7_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001172 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001172 --> ing9_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing10_NaOH["NaOH"]
    CultureMech_001172 --> ing10_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing10_NaOH -.-> CHEBI_32145
    ing11_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001172 --> ing11_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing11_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing12_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001172 --> ing12_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing12_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing13_Ammonium_chloride["Ammonium chloride"]
    CultureMech_001172 --> ing13_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing13_Ammonium_chloride -.-> CHEBI_31206
    ing14_KH2PO4["KH2PO4"]
    CultureMech_001172 --> ing14_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing14_KH2PO4 -.-> CHEBI_63036
    ing15_KCl["KCl"]
    CultureMech_001172 --> ing15_KCl
    CHEBI_32588["CHEBI:32588"]
    ing15_KCl -.-> CHEBI_32588
    ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001172 --> ing16_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing17_NaCl["NaCl"]
    CultureMech_001172 --> ing17_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing17_NaCl -.-> CHEBI_26710

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 CaCO3
CO3.Ca 100.086 g/mol
4 G_PER_L CHEBI:3311
calcium carbonate
2 H3BO3
H3BO3 61.833 g/mol
0.06 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.054, 0.006]
3 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.019999999999999997 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 [Merged 2 duplicates: 0.018, 0.002]
4 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
5 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
6 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
10 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
11 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
12 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
13 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
14 Ammonium chloride
Cl.H4N 53.492 g/mol
8 G_PER_L CHEBI:31206
ammonium chloride
15 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
16 KCl
Cl.K 74.551 g/mol
1.5 G_PER_L CHEBI:32588
potassium chloride
17 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
18 NaCl
Cl.Na 58.443 g/mol
11.68 G_PER_L CHEBI:26710
sodium chloride

Preparation

  1. Dilute stock solution with MilliQ water (1+19 v/v), add 4 g CaCO3 per litre.
  2. Autoclave at 120 °C for 21 min.
  3. Cool down to room temperature.
  4. After autoclaving add sterile solutions:
  5. Check pH 7.6-8.0
  6. Complete medium can be stored at 4°C.
  7. Incubate at 42°C without shaking. Activity and growth can be followed by measuring the depletion of ammonium or the production of nitrite and/or nitrate. Higher ammonium concentrations are inhibitive. For that reason our recommendation is follow nitrite content. As soon as nitrite has been produced and is depleting again (to form nitrate) another dose of ammonium can be added. Incubation time may be up to several weeks. We checked the nitrite content (absence/presence) every 5-7 days and added ammonium chloride about every 2-3 weeks. Don't expose cultures to intense light.
  8. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
  9. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MQ water.
  10. Autoclave at 120 °C for 21 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.249480Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1690
2026-03-13T03:09:23.221608Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:01.884215Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001172
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.653836+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NITROSPIRA INOPINATA MEDIUM" to "nitrospira_inopinata_medium" for consistent matching
2026-05-20T06:02:01.163884+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.6-8' -> {'min': 7.6, 'max': 8.0}
2026-06-05T07:41:39.925429+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.641984+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.