Overview
- External ontology
- mediadive.medium:1694 (source link unavailable) — Acidobacteriaceae Medium (Modified VSB Medium)
- Applications
-
- Microbial cultivation
- pH
- 5.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1694.pdf
Composition
flowchart LR
CultureMech_001175["acidobacteriaceae_medium_modified_vsb_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001175 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_001175 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KCl["KCl"]
CultureMech_001175 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001175 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NaCl["NaCl"]
CultureMech_001175 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001175 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_Yeast_extract["Yeast extract"]
CultureMech_001175 --> ing6_Yeast_extract
ing7_Proteose_peptone["Proteose peptone"]
CultureMech_001175 --> ing7_Proteose_peptone
ing8_Casamino_acids["Casamino acids"]
CultureMech_001175 --> ing8_Casamino_acids
ing9_Glucose["Glucose"]
CultureMech_001175 --> ing9_Glucose
CHEBI_17234["CHEBI:17234"]
ing9_Glucose -.-> CHEBI_17234
ing10_HEPES["HEPES"]
CultureMech_001175 --> ing10_HEPES
CHEBI_46756["CHEBI:46756"]
ing10_HEPES -.-> CHEBI_46756
ing11_Na2SO4["Na2SO4"]
CultureMech_001175 --> ing11_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing11_Na2SO4 -.-> CHEBI_32149
ing12_Vitamin_B12["Vitamin B12"]
CultureMech_001175 --> ing12_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing12_Vitamin_B12 -.-> CHEBI_176843
ing13_HCl["HCl"]
CultureMech_001175 --> ing13_HCl
CHEBI_17883["CHEBI:17883"]
ing13_HCl -.-> CHEBI_17883
ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001175 --> ing14_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
ing15_ZnCl2["ZnCl2"]
CultureMech_001175 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001175 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_H3BO3["H3BO3"]
CultureMech_001175 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001175 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001175 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001175 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001175 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_Na_Phosphate_Buffer["Na-Phosphate-Buffer"]
CultureMech_001175 --> ing22_Na_Phosphate_Buffer
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001175 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_D_____biotin["D-(+)-biotin"]
CultureMech_001175 --> ing24_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing24_D_____biotin -.-> CHEBI_15956
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_001175 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_001175 --> ing26_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_D_Calcium_pantothenate -.-> CHEBI_31345
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001175 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.62 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 | Yeast extract | 0.15 G_PER_L | ||
| 8 | Proteose peptone | 0.15 G_PER_L | ||
| 9 | Casamino acids | 0.15 G_PER_L | ||
| 10 |
Glucose
C6H12O6 180.156 g/mol
|
0.45 G_PER_L |
CHEBI:17234
glucose
|
|
| 11 | HEPES | 2.383 G_PER_L |
CHEBI:46756
HEPES
|
|
| 12 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.8408 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 13 | Vitamin B12 | 0.5 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 14 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 15 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 | Na-Phosphate-Buffer | 100 G_PER_L | ||
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.04 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 25 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.1 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.15 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Preparation
- adjust pH to 5.0
- Add to 1000 ml of medium after autoclaving:
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- acidobacteriaceae_medium_modified_vsb_medium (CultureMech:001175)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.260019Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1694 |
| 2026-01-28T19:48:42.346245Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:01.915648Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001175 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.689288+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Acidobacteriaceae Medium (Modified VSB Medium)" to "acidobacteriaceae_medium_modified_vsb_medium" for consistent matching |
| 2026-06-05T07:40:47.390039+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.489097+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:52.629139+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.057823+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 476c0fbe2f3f84bdef2c28840ee24b208783d5a472d8e3732cbc5dffaf6b797e |