acidobacteriaceae_medium_modified_vsb_medium

CultureMech:001175 bacterial COMPLEX LIQUID
Original name: Acidobacteriaceae Medium (Modified VSB Medium)

Overview

External ontology
mediadive.medium:1694 (source link unavailable) — Acidobacteriaceae Medium (Modified VSB Medium)
Applications
  • Microbial cultivation
pH
5.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1694.pdf

Composition

flowchart LR
    CultureMech_001175["acidobacteriaceae_medium_modified_vsb_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001175 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001175 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KCl["KCl"]
    CultureMech_001175 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001175 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NaCl["NaCl"]
    CultureMech_001175 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001175 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001175 --> ing6_Yeast_extract
    ing7_Proteose_peptone["Proteose peptone"]
    CultureMech_001175 --> ing7_Proteose_peptone
    ing8_Casamino_acids["Casamino acids"]
    CultureMech_001175 --> ing8_Casamino_acids
    ing9_Glucose["Glucose"]
    CultureMech_001175 --> ing9_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing9_Glucose -.-> CHEBI_17234
    ing10_HEPES["HEPES"]
    CultureMech_001175 --> ing10_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing10_HEPES -.-> CHEBI_46756
    ing11_Na2SO4["Na2SO4"]
    CultureMech_001175 --> ing11_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing11_Na2SO4 -.-> CHEBI_32149
    ing12_Vitamin_B12["Vitamin B12"]
    CultureMech_001175 --> ing12_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing12_Vitamin_B12 -.-> CHEBI_176843
    ing13_HCl["HCl"]
    CultureMech_001175 --> ing13_HCl
    CHEBI_17883["CHEBI:17883"]
    ing13_HCl -.-> CHEBI_17883
    ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001175 --> ing14_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing15_ZnCl2["ZnCl2"]
    CultureMech_001175 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001175 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_H3BO3["H3BO3"]
    CultureMech_001175 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001175 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001175 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001175 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001175 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_Na_Phosphate_Buffer["Na-Phosphate-Buffer"]
    CultureMech_001175 --> ing22_Na_Phosphate_Buffer
    ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001175 --> ing23_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing24_D_____biotin["D-(+)-biotin"]
    CultureMech_001175 --> ing24_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing24_D_____biotin -.-> CHEBI_15956
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001175 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_001175 --> ing26_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_D_Calcium_pantothenate -.-> CHEBI_31345
    ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001175 --> ing27_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
3 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.62 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 Yeast extract 0.15 G_PER_L
8 Proteose peptone 0.15 G_PER_L
9 Casamino acids 0.15 G_PER_L
10 Glucose
C6H12O6 180.156 g/mol
0.45 G_PER_L CHEBI:17234
glucose
11 HEPES 2.383 G_PER_L CHEBI:46756
HEPES
12 Na2SO4
2Na.O4S 142.043 g/mol
2.8408 G_PER_L CHEBI:32149
sodium sulfate
13 Vitamin B12 0.5 G_PER_L CHEBI:176843
vitamin B12
14 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
15 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
17 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 Na-Phosphate-Buffer 100 G_PER_L
24 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.04 G_PER_L CHEBI:30753
4-aminobenzoic acid
25 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.01 G_PER_L CHEBI:15956
biotin
26 Nicotinic acid
C6H5NO2 123.111 g/mol
0.1 G_PER_L CHEBI:15940
nicotinic acid
27 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
28 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.15 G_PER_L CHEBI:30961
pyridoxine hydrochloride

Preparation

  1. adjust pH to 5.0
  2. Add to 1000 ml of medium after autoclaving:
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.260019Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1694
2026-01-28T19:48:42.346245Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:01.915648Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001175
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.689288+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Acidobacteriaceae Medium (Modified VSB Medium)" to "acidobacteriaceae_medium_modified_vsb_medium" for consistent matching
2026-06-05T07:40:47.390039+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:35.489097+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:52.629139+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.