Overview
- External ontology
- mediadive.medium:169b (source link unavailable) — LEADBETTERA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium169b.pdf
Composition
flowchart LR
CultureMech_001181["leadbettera_medium"]
ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001181 --> ing0_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001181 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_001181 --> ing2_Yeast_extract
ing3_D_Glucose["D-Glucose"]
CultureMech_001181 --> ing3_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing3_D_Glucose -.-> CHEBI_17634
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_001181 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001181 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_NaHCO3["NaHCO3"]
CultureMech_001181 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_K2HPO4["K2HPO4"]
CultureMech_001181 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_KH2PO4["KH2PO4"]
CultureMech_001181 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_DL_Dithiothreitol["DL-Dithiothreitol"]
CultureMech_001181 --> ing9_DL_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing9_DL_Dithiothreitol -.-> CHEBI_18320
ing10_HCl["HCl"]
CultureMech_001181 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001181 --> ing11_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
ing12_ZnCl2["ZnCl2"]
CultureMech_001181 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001181 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_001181 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001181 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001181 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001181 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001181 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_NaOH["NaOH"]
CultureMech_001181 --> ing19_NaOH
CHEBI_32145["CHEBI:32145"]
ing19_NaOH -.-> CHEBI_32145
ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001181 --> ing20_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001181 --> ing21_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_001181 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001181 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_D_____biotin["D-(+)-biotin"]
CultureMech_001181 --> ing24_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing24_D_____biotin -.-> CHEBI_15956
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_001181 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001181 --> ing26_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_pantothenate -.-> CHEBI_31345
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001181 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing28_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001181 --> ing28_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing28_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0420168 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.52521 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 | Yeast extract | 4.20168 G_PER_L | ||
| 4 |
D-Glucose
C6H12O6 180.156 g/mol
|
4.20168 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.52521 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
50 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
16.04 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3.96 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
DL-Dithiothreitol
C4H10O2S2 154.256 g/mol
|
15 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 11 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 12 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 21 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 22 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 23 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 25 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 29 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- Dissolve ingredients of solution A (except cysteine) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH 7.2 with KOH, then distribute under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere, solution C is autoclaved under 100% N2 gas atmosphere and solutions D and E are sterilized by filtration under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B, C, D and E are added to the sterile solution A in the sequence as indicated. Final pH of the complete medium should be 7.5.
- Combine 16.04 ml of 0.5 M K2HPO4 with 3.96 ml of 0.5 M KH2PO4 stock solution to reach a pH of around 7.4.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- leadbettera_medium (CultureMech:001181)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.392296Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 169b |
| 2026-03-13T06:19:01.977714Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001181 |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.757250+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LEADBETTERA MEDIUM" to "leadbettera_medium" for consistent matching |
| 2026-06-05T07:41:11.006995+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.507776+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:40.110179+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 261f3563ffe61770ba526c9b342ff47aa20c071d70768d8c82bee5b98e5763a1 |