Overview
- External ontology
- mediadive.medium:169b (source link unavailable) — LEADBETTERA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium169b.pdf
Composition
flowchart LR
CultureMech_001181["leadbettera_medium"]
ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001181 --> ing0_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001181 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_001181 --> ing2_Yeast_extract
ing3_D_Glucose["D-Glucose"]
CultureMech_001181 --> ing3_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing3_D_Glucose -.-> CHEBI_17634
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_001181 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001181 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_NaHCO3["NaHCO3"]
CultureMech_001181 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_K2HPO4["K2HPO4"]
CultureMech_001181 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_KH2PO4["KH2PO4"]
CultureMech_001181 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_DL_Dithiothreitol["DL-Dithiothreitol"]
CultureMech_001181 --> ing9_DL_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing9_DL_Dithiothreitol -.-> CHEBI_18320
ing10_HCl["HCl"]
CultureMech_001181 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
ing11_ZnCl2["ZnCl2"]
CultureMech_001181 --> ing11_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing11_ZnCl2 -.-> CHEBI_49976
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001181 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_H3BO3["H3BO3"]
CultureMech_001181 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001181 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001181 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001181 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001181 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_NaOH["NaOH"]
CultureMech_001181 --> ing18_NaOH
CHEBI_32145["CHEBI:32145"]
ing18_NaOH -.-> CHEBI_32145
ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001181 --> ing19_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001181 --> ing20_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001181 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_D_____biotin["D-(+)-biotin"]
CultureMech_001181 --> ing22_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing22_D_____biotin -.-> CHEBI_15956
ing23_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_001181 --> ing23_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing23_Calcium_pantothenate -.-> CHEBI_31345
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0420168 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.52521 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 | Yeast extract | 4.20168 G_PER_L | ||
| 4 |
D-Glucose
C6H12O6 180.156 g/mol
|
4.20168 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.52521 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
50 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
16.04 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3.96 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
DL-Dithiothreitol
C4H10O2S2 154.256 g/mol
|
15 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 11 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 12 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 20 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 21 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Preparation
- Dissolve ingredients of solution A (except cysteine) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH 7.2 with KOH, then distribute under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere, solution C is autoclaved under 100% N2 gas atmosphere and solutions D and E are sterilized by filtration under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B, C, D and E are added to the sterile solution A in the sequence as indicated. Final pH of the complete medium should be 7.5.
- Combine 16.04 ml of 0.5 M K2HPO4 with 3.96 ml of 0.5 M KH2PO4 stock solution to reach a pH of around 7.4.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solutions
Trace element solution SL-10
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl2 x 4 H2O | 1.5 G_PER_L |
Seven vitamins solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Vitamin B12 | 0.1 G_PER_L | |
| Nicotinic acid | 0.2 G_PER_L | |
| Pyridoxine hydrochloride | 0.3 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.2 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.392296Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 169b |
| 2026-03-13T06:19:01.977714Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001181 |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.757250+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LEADBETTERA MEDIUM" to "leadbettera_medium" for consistent matching |
| 2026-06-05T07:41:11.006995+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.507776+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T07:05:22.418916+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace element solution SL-10' @ 1 ml/l; 4 -> 'Seven vitamins solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |