Overview
- External ontology
- mediadive.medium:1723 (source link unavailable) — Enriched Artificial Seawater Medium f/2
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1723.pdf
Composition
flowchart LR
CultureMech_001202["enriched_artificial_seawater_medium_f_2"]
ing0_Artificial_Sea_Salt["Artificial Sea Salt"]
CultureMech_001202 --> ing0_Artificial_Sea_Salt
ing1_NaNO3["NaNO3"]
CultureMech_001202 --> ing1_NaNO3
CHEBI_63005["CHEBI:63005"]
ing1_NaNO3 -.-> CHEBI_63005
ing2_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
CultureMech_001202 --> ing2_NaH2PO4_x_H2O
CHEBI_37585["CHEBI:37585"]
ing2_NaH2PO4_x_H2O -.-> CHEBI_37585
ing3_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_001202 --> ing3_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing3_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_001202 --> ing4_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001202 --> ing5_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001202 --> ing6_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001202 --> ing7_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001202 --> ing8_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing8_CuSO4_x_5_H2O -.-> CHEBI_31440
ing9_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
CultureMech_001202 --> ing9_Na2_MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
ing10_Thiamine_HCl["Thiamine HCl"]
CultureMech_001202 --> ing10_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing10_Thiamine_HCl -.-> CHEBI_49105
ing11_D_Biotin["D-Biotin"]
CultureMech_001202 --> ing11_D_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_D_Biotin -.-> CHEBI_15956
ing12_Vitamin_B12["Vitamin B12"]
CultureMech_001202 --> ing12_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing12_Vitamin_B12 -.-> CHEBI_176843
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Artificial Sea Salt | 12.4 G_PER_L | (~ 18 mS/cm = ~ 12 psu at 25°C) | |
| 2 |
NaNO3
NO3.Na 84.994 g/mol
|
0.075 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 3 |
NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
|
0.005 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 4 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
4.16 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 5 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
3.15 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 6 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
45 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 7 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.02196 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 8 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.7 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 9 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.8 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 10 |
Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
2.2 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 11 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 12 |
D-Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 | Vitamin B12 | 0.25 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Optional: To prepare solid medium, use 7 g/l agarose. Sterilize the agarose separately in half of the MilliQ water and combine the agarose solution and remaining medium (1. & 2.) after autoclaving.
- Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
- Autoclave at 121 °C for 20 min.
- After cooling, add the following filter sterilized (0.2 µm) components to complete the medium:
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- enriched_artificial_seawater_medium_f_2 (CultureMech:001202)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.359708Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1723 |
| 2026-03-13T06:19:02.191616Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001202 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:13.998097+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Enriched Artificial Seawater Medium f/2" to "enriched_artificial_seawater_medium_f_2" for consistent matching |
| 2026-06-05T07:40:59.589113+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:33.620554+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d60f153066dac94ced04e2de51946e4c6fe2cb5945da13b7b2dfbcc7301c1f67 |