enriched_artificial_seawater_medium_f_2 (CultureMech:001202)

Overview

External ontology

mediadive.medium:1723 (source link unavailable) — Enriched Artificial Seawater Medium f/2

Applications

Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1723.pdf

Composition

flowchart LR
    CultureMech_001202["enriched_artificial_seawater_medium_f_2"]
    ing0_Artificial_Sea_Salt["Artificial Sea Salt"]
    CultureMech_001202 --> ing0_Artificial_Sea_Salt
    ing1_NaNO3["NaNO3"]
    CultureMech_001202 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
    CultureMech_001202 --> ing2_NaH2PO4_x_H2O
    CHEBI_37585["CHEBI:37585"]
    ing2_NaH2PO4_x_H2O -.-> CHEBI_37585
    ing3_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_001202 --> ing3_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing3_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing4_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001202 --> ing4_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing4_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing5_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001202 --> ing5_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing5_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing6_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_001202 --> ing6_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing6_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing7_D_Biotin["D-Biotin"]
    CultureMech_001202 --> ing7_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing7_D_Biotin -.-> CHEBI_15956

Ingredients (8)

#	Ingredient	Concentration	Ontology	Notes
1	Artificial Sea Salt	12.4 G_PER_L		(~ 18 mS/cm = ~ 12 psu at 25°C)
2	NaNO3 NO3.Na 84.994 g/mol	0.075 G_PER_L	CHEBI:63005 sodium nitrate
3	NaH2PO4 x H2O H2O4P.Na 119.976 g/mol	0.005 G_PER_L	CHEBI:37585 sodium dihydrogenphosphate
4	Na2-EDTA x 2 H2O C10H14N2O8.2H2O.2Na 372.238 g/mol	4.16 G_PER_L	CHEBI:64758 EDTA disodium salt dihydrate
5	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.02196 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate
6	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.8 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate
7	Na2 MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	2.2 G_PER_L	CHEBI:75213 sodium molybdate dihydrate
8	D-Biotin C10H16N2O3S 244.316 g/mol	0.1 G_PER_L	CHEBI:15956 biotin

Preparation

Optional: To prepare solid medium, use 7 g/l agarose. Sterilize the agarose separately in half of the MilliQ water and combine the agarose solution and remaining medium (1. & 2.) after autoclaving.
Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
Autoclave at 121 °C for 20 min.
After cooling, add the following filter sterilized (0.2 µm) components to complete the medium:

Solutions

f/2 Trace Metal Mix

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
FeCl3 x 6 H2O	3.15 G_PER_L
MnCl2 x 4 H2O	45 G_PER_L
CoCl2 x 6 H2O	1.7 G_PER_L

Vitamin 3 Mix

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Thiamine HCl	1 G_PER_L
Vitamin B12	0.25 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:15.359708Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1723
2026-03-13T06:19:02.191616Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001202
2026-03-17T06:30:12.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:13.998097+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Enriched Artificial Seawater Medium f/2" to "enriched_artificial_seawater_medium_f_2" for consistent matching
2026-06-05T07:40:59.589113+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-07T07:05:22.183634+00:00	apply_cocktail_nesting.py	NESTED_FLATTENED_COCKTAIL	Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 3 -> 'f/2 Trace Metal Mix' @ 1 ml/l; 2 -> 'Vitamin 3 Mix' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).