enriched_artificial_seawater_medium_f_2_suppl_with_wheat_grains

CultureMech:001203 bacterial COMPLEX LIQUID
Original name: Enriched Artificial Seawater Medium f/2 suppl. with Wheat Grains

Overview

External ontology
mediadive.medium:1723a (source link unavailable) — Enriched Artificial Seawater Medium f/2 suppl. with Wheat Grains
Applications
  • Microbial cultivation
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001203["enriched_artificial_seawater_medium_f_2_suppl_with_wheat_grains"]
    ing0_Artificial_Sea_Salt["Artificial Sea Salt"]
    CultureMech_001203 --> ing0_Artificial_Sea_Salt
    ing1_NaNO3["NaNO3"]
    CultureMech_001203 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
    CultureMech_001203 --> ing2_NaH2PO4_x_H2O
    CHEBI_37585["CHEBI:37585"]
    ing2_NaH2PO4_x_H2O -.-> CHEBI_37585
    ing3_Wheat_Grains["Wheat Grains"]
    CultureMech_001203 --> ing3_Wheat_Grains
    ing4_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_001203 --> ing4_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing4_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001203 --> ing5_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001203 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001203 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001203 --> ing8_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001203 --> ing9_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing10_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_001203 --> ing10_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001203 --> ing11_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing11_Thiamine_HCl -.-> CHEBI_49105
    ing12_D_Biotin["D-Biotin"]
    CultureMech_001203 --> ing12_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing12_D_Biotin -.-> CHEBI_15956
    ing13_Vitamin_B12["Vitamin B12"]
    CultureMech_001203 --> ing13_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing13_Vitamin_B12 -.-> CHEBI_176843

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Artificial Sea Salt 12.4 G_PER_L (~ 18 mS/cm = ~ 12 psu at 25°C)
2 NaNO3
NO3.Na 84.994 g/mol
0.075 G_PER_L CHEBI:63005
sodium nitrate
3 NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
0.005 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
4 Wheat Grains variable VARIABLE
5 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
4.16 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
6 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
3.15 G_PER_L CHEBI:86254
iron trichloride hexahydrate
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
45 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.02196 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
1.7 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
10 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.8 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
11 Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
2.2 G_PER_L CHEBI:75213
sodium molybdate dihydrate
12 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
1 G_PER_L CHEBI:49105
thiamine hydrochloride
13 D-Biotin
C10H16N2O3S 244.316 g/mol
0.1 G_PER_L CHEBI:15956
biotin
14 Vitamin B12 0.25 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
  2. Autoclave at 121 °C for 20 min.
  3. After cooling, add the following filter sterilized (0.2 µm) components to complete the medium:
  4. The strain is cultivated in 25 cm2 plastic tissue culture flasks with filter screw caps (T25, distributor: TPP, order number: 90026). The T25 flasks are routinely filled with 20 ml of culture. Each T25 culture is supplemented with a sterilized wheat grain as carbon source for ambient bacteria from the sampling site.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.356783Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1723a
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Wheat Grains
2026-03-13T06:19:02.199225Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001203
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.006770+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Enriched Artificial Seawater Medium f/2 suppl. with Wheat Grains" to "enriched_artificial_seawater_medium_f_2_suppl_with_wheat_grains" for consistent matching
2026-05-16T09:03:31.501955+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:59.608483+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:33.641289+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint c06516e53278476887bb1b7b0c16e8a22dac49d26fd4035c62018e2cd1deb146