enriched_artificial_seawater_medium_f_2_suppl_with_wheat_grains

CultureMech:001203 bacterial COMPLEX LIQUID
Original name: Enriched Artificial Seawater Medium f/2 suppl. with Wheat Grains

Overview

External ontology
mediadive.medium:1723a (source link unavailable) — Enriched Artificial Seawater Medium f/2 suppl. with Wheat Grains
Applications
  • Microbial cultivation
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001203["enriched_artificial_seawater_medium_f_2_suppl_with_wheat_grains"]
    ing0_Artificial_Sea_Salt["Artificial Sea Salt"]
    CultureMech_001203 --> ing0_Artificial_Sea_Salt
    ing1_NaNO3["NaNO3"]
    CultureMech_001203 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
    CultureMech_001203 --> ing2_NaH2PO4_x_H2O
    CHEBI_37585["CHEBI:37585"]
    ing2_NaH2PO4_x_H2O -.-> CHEBI_37585
    ing3_Wheat_Grains["Wheat Grains"]
    CultureMech_001203 --> ing3_Wheat_Grains
    ing4_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_001203 --> ing4_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing4_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing5_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001203 --> ing5_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing5_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing6_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001203 --> ing6_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing6_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing7_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_001203 --> ing7_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing7_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing8_D_Biotin["D-Biotin"]
    CultureMech_001203 --> ing8_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_D_Biotin -.-> CHEBI_15956

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Artificial Sea Salt 12.4 G_PER_L (~ 18 mS/cm = ~ 12 psu at 25°C)
2 NaNO3
NO3.Na 84.994 g/mol
0.075 G_PER_L CHEBI:63005
sodium nitrate
3 NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
0.005 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
4 Wheat Grains variable VARIABLE
5 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
4.16 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
6 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.02196 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
7 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.8 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
8 Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
2.2 G_PER_L CHEBI:75213
sodium molybdate dihydrate
9 D-Biotin
C10H16N2O3S 244.316 g/mol
0.1 G_PER_L CHEBI:15956
biotin

Preparation

  1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
  2. Autoclave at 121 °C for 20 min.
  3. After cooling, add the following filter sterilized (0.2 µm) components to complete the medium:
  4. The strain is cultivated in 25 cm2 plastic tissue culture flasks with filter screw caps (T25, distributor: TPP, order number: 90026). The T25 flasks are routinely filled with 20 ml of culture. Each T25 culture is supplemented with a sterilized wheat grain as carbon source for ambient bacteria from the sampling site.

Solutions

f/2 Trace Metal Mix

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl3 x 6 H2O 3.15 G_PER_L
MnCl2 x 4 H2O 45 G_PER_L
CoCl2 x 6 H2O 1.7 G_PER_L

Vitamin 3 Mix

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Thiamine HCl 1 G_PER_L
Vitamin B12 0.25 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.356783Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1723a
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Wheat Grains
2026-03-13T06:19:02.199225Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001203
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.006770+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Enriched Artificial Seawater Medium f/2 suppl. with Wheat Grains" to "enriched_artificial_seawater_medium_f_2_suppl_with_wheat_grains" for consistent matching
2026-05-16T09:03:31.501955+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:59.608483+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-07T07:05:22.224401+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 3 -> 'f/2 Trace Metal Mix' @ 1 ml/l; 2 -> 'Vitamin 3 Mix' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).