Overview
- External ontology
- mediadive.medium:1749 (source link unavailable) — Sucrose-Asparagine Medium
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1749.pdf
Composition
flowchart LR
CultureMech_001222["sucrose_asparagine_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001222 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_K2HPO4["K2HPO4"]
CultureMech_001222 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_L_Asparagin["L-Asparagin"]
CultureMech_001222 --> ing2_L_Asparagin
CHEBI_17196["CHEBI:17196"]
ing2_L_Asparagin -.-> CHEBI_17196
ing3_Tap_water["Tap water"]
CultureMech_001222 --> ing3_Tap_water
CHEBI_15377["CHEBI:15377"]
ing3_Tap_water -.-> CHEBI_15377
ing4_Agar["Agar"]
CultureMech_001222 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Sucrose["Sucrose"]
CultureMech_001222 --> ing5_Sucrose
CHEBI_17992["CHEBI:17992"]
ing5_Sucrose -.-> CHEBI_17992
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.50505 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.0101 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
L-Asparagin
C4H8N2O3 132.119 g/mol
|
2.0202 G_PER_L |
CHEBI:17196
L-asparagine
|
|
| 4 |
Tap water
H2O 18.015 g/mol
|
990 G_PER_L |
CHEBI:15377
water
|
|
| 5 | Agar | 15.1515 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 6 |
Sucrose
C12H22O11 342.297 g/mol
|
2000 G_PER_L |
CHEBI:17992
sucrose
|
Preparation
- Adjust to pH 7.0 sterilize by autoclaving. The medium may be solidified by adding 15.0 g/l agar.
- Sterilize by autoclaving.
- After autoclaving add 10 mL of the Sucrose solution to the Mineral Salts Solution with Asparagine (final volume 1 L).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sucrose_asparagine_medium (CultureMech:001222)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.425164Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1749 |
| 2026-03-13T06:19:02.384256Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001222 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.207607+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sucrose-Asparagine Medium" to "sucrose_asparagine_medium" for consistent matching |
| 2026-06-05T07:41:47.226881+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.928172+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.664365+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a17b7562b56882f9d3bc35b1bc89c1de94eec38dc6c9a88cf6a5ce856f15f12d |