sucrose_asparagine_medium

CultureMech:001222 bacterial DEFINED SOLID_AGAR
Original name: Sucrose-Asparagine Medium

Overview

External ontology
mediadive.medium:1749 (source link unavailable) — Sucrose-Asparagine Medium
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1749.pdf

Composition

flowchart LR
    CultureMech_001222["sucrose_asparagine_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001222 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001222 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_L_Asparagin["L-Asparagin"]
    CultureMech_001222 --> ing2_L_Asparagin
    CHEBI_17196["CHEBI:17196"]
    ing2_L_Asparagin -.-> CHEBI_17196
    ing3_Tap_water["Tap water"]
    CultureMech_001222 --> ing3_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Tap_water -.-> CHEBI_15377
    ing4_Agar["Agar"]
    CultureMech_001222 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_Sucrose["Sucrose"]
    CultureMech_001222 --> ing5_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing5_Sucrose -.-> CHEBI_17992

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.50505 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 K2HPO4
HO4P.2K 174.174 g/mol
1.0101 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 L-Asparagin
C4H8N2O3 132.119 g/mol
2.0202 G_PER_L CHEBI:17196
L-asparagine
4 Tap water
H2O 18.015 g/mol
990 G_PER_L CHEBI:15377
water
5 Agar 15.1515 G_PER_L CHEBI:2509
agar
 (for solid medium)
6 Sucrose
C12H22O11 342.297 g/mol
2000 G_PER_L CHEBI:17992
sucrose

Preparation

  1. Adjust to pH 7.0 sterilize by autoclaving. The medium may be solidified by adding 15.0 g/l agar.
  2. Sterilize by autoclaving.
  3. After autoclaving add 10 mL of the Sucrose solution to the Mineral Salts Solution with Asparagine (final volume 1 L).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.425164Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1749
2026-03-13T06:19:02.384256Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001222
2026-03-17T06:30:30.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.207607+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Sucrose-Asparagine Medium" to "sucrose_asparagine_medium" for consistent matching
2026-06-05T07:41:47.226881+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.928172+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.