mwc

CultureMech:001255 bacterial DEFINED LIQUID
Original name: MWC

Overview

External ontology
mediadive.medium:1828 (source link unavailable) — MWC
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 8.0}
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001255["mwc"]
    ing0_TES["TES"]
    CultureMech_001255 --> ing0_TES
    CHEBI_39035["CHEBI:39035"]
    ing0_TES -.-> CHEBI_39035
    ing1_NaNO3["NaNO3"]
    CultureMech_001255 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001255 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001255 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001255 --> ing4_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing5_NaHCO3["NaHCO3"]
    CultureMech_001255 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_Na2SiO3_x_5_H2O["Na2SiO3 x 5 H2O"]
    CultureMech_001255 --> ing6_Na2SiO3_x_5_H2O
    CHEBI_60720["CHEBI:60720"]
    ing6_Na2SiO3_x_5_H2O -.-> CHEBI_60720
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_001255 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001255 --> ing8_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing8_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing9_H3BO3["H3BO3"]
    CultureMech_001255 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001255 --> ing10_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001255 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001255 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001255 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001255 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001255 --> ing15_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing15_Thiamine_HCl -.-> CHEBI_49105
    ing16_Biotin["Biotin"]
    CultureMech_001255 --> ing16_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing16_Biotin -.-> CHEBI_15956
    ing17_Vitamin_B12["Vitamin B12"]
    CultureMech_001255 --> ing17_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing17_Vitamin_B12 -.-> CHEBI_176843

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 TES 0.115 G_PER_L CHEBI:39035
TES
2 NaNO3
NO3.Na 84.994 g/mol
2 G_PER_L CHEBI:63005
sodium nitrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
2.62 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 NaHCO3
CHO3.Na 84.006 g/mol
0.4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 Na2SiO3 x 5 H2O
2Na.O3Si 122.063 g/mol
1 G_PER_L CHEBI:60720
sodium silicate
8 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
4.36 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
9 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
3.15 G_PER_L CHEBI:86254
iron trichloride hexahydrate
10 H3BO3
H3BO3 61.833 g/mol
1 G_PER_L CHEBI:33118
boric acid
11 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.8 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
12 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
1.667 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
2.222 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.1 G_PER_L CHEBI:49105
thiamine hydrochloride
17 Biotin
C10H16N2O3S 244.316 g/mol
0.1 G_PER_L CHEBI:15956
biotin
18 Vitamin B12 0.25 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
  2. Autoclave at 121 °C for 20 min.
  3. After cooling add the following filter sterilized component under sterile conditions:
  4. Adjust pH to 4.0-4.5.
  5. Make up to <span class='amount' data-volume='1000'>1000</span> ml with distilled water.
  6. Make up to <span class='amount' data-volume='100'>100</span> ml with MilliQ water.
  7. Filter sterilise, aliquot and store frozen.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:15.499250Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1828
2026-03-13T06:19:02.590794Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001255
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.431796+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MWC" to "mwc" for consistent matching
2026-05-20T06:00:47.124050+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-8' -> {'min': 7.0, 'max': 8.0}
2026-06-05T07:40:16.131966+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.059571+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:42.629885+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:34.590546+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: DSMZ_1828_MWC, mwc
2026-08-06T03:04:34.590585+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint f5ba97aee8eb87fdd6a2836fe2764519613767073586bfacbf9f68a93b2aae01