mwc

CultureMech:001255 bacterial DEFINED LIQUID
Original name: MWC

Overview

External ontology
mediadive.medium:1828 (source link unavailable) — MWC
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 8.0}
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001255["mwc"]
    ing0_TES["TES"]
    CultureMech_001255 --> ing0_TES
    CHEBI_39035["CHEBI:39035"]
    ing0_TES -.-> CHEBI_39035
    ing1_NaNO3["NaNO3"]
    CultureMech_001255 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001255 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001255 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001255 --> ing4_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing5_NaHCO3["NaHCO3"]
    CultureMech_001255 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_Na2SiO3_x_5_H2O["Na2SiO3 x 5 H2O"]
    CultureMech_001255 --> ing6_Na2SiO3_x_5_H2O
    CHEBI_60720["CHEBI:60720"]
    ing6_Na2SiO3_x_5_H2O -.-> CHEBI_60720
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_001255 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001255 --> ing8_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing8_CuSO4_x_5_H2O -.-> CHEBI_31440

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 TES 0.115 G_PER_L CHEBI:39035
TES
2 NaNO3
NO3.Na 84.994 g/mol
2 G_PER_L CHEBI:63005
sodium nitrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
2.62 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 NaHCO3
CHO3.Na 84.006 g/mol
0.4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 Na2SiO3 x 5 H2O
2Na.O3Si 122.063 g/mol
1 G_PER_L CHEBI:60720
sodium silicate
8 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
4.36 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
9 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.8 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate

Preparation

  1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
  2. Autoclave at 121 °C for 20 min.
  3. After cooling add the following filter sterilized component under sterile conditions:
  4. Adjust pH to 4.0-4.5.
  5. Make up to <span class='amount' data-volume='1000'>1000</span> ml with distilled water.
  6. Make up to <span class='amount' data-volume='100'>100</span> ml with MilliQ water.
  7. Filter sterilise, aliquot and store frozen.

Solutions

Combined trace element solution*

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl3 x 6 H2O 3.15 G_PER_L
H3BO3 1 G_PER_L
ZnSO4 x 7 H2O 1 G_PER_L
CoCl2 x 6 H2O 1.667 G_PER_L
MnCl2 x 4 H2O 1 G_PER_L
Na2MoO4 x 2 H2O 2.222 G_PER_L

MWC Vitamin mix**

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Thiamine HCl 0.1 G_PER_L
Biotin 0.1 G_PER_L
Vitamin B12 0.25 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.499250Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1828
2026-03-13T06:19:02.590794Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001255
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.431796+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MWC" to "mwc" for consistent matching
2026-05-20T06:00:47.124050+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-8' -> {'min': 7.0, 'max': 8.0}
2026-06-05T07:40:16.131966+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.059571+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:42.629885+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:21.540440+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 9 stock-strength component(s) out of ingredients into 2 solution(s): 6 -> 'Combined trace element solution*' @ 1 ml/l; 3 -> 'MWC Vitamin mix**' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).