Overview
- External ontology
- mediadive.medium:1829 (source link unavailable) — BSKII medium (Borrelia)
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: DSMZ Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001256["bskii_medium_borrelia"]
ing0_HEPES["HEPES"]
CultureMech_001256 --> ing0_HEPES
CHEBI_46756["CHEBI:46756"]
ing0_HEPES -.-> CHEBI_46756
ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_001256 --> ing1_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing2_Glucose["Glucose"]
CultureMech_001256 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Pyruvate["Pyruvate"]
CultureMech_001256 --> ing3_Pyruvate
CHEBI_15361["CHEBI:15361"]
ing3_Pyruvate -.-> CHEBI_15361
ing4_N_Acetylglucosamine["N-Acetylglucosamine"]
CultureMech_001256 --> ing4_N_Acetylglucosamine
CHEBI_59640["CHEBI:59640"]
ing4_N_Acetylglucosamine -.-> CHEBI_59640
ing5_Sodium_bicarbonate["Sodium bicarbonate"]
CultureMech_001256 --> ing5_Sodium_bicarbonate
CHEBI_32139["CHEBI:32139"]
ing5_Sodium_bicarbonate -.-> CHEBI_32139
ing6_Yeast_extract["Yeast extract"]
CultureMech_001256 --> ing6_Yeast_extract
ing7_Bacto_peptone["Bacto peptone"]
CultureMech_001256 --> ing7_Bacto_peptone
ing8_Tryptone["Tryptone"]
CultureMech_001256 --> ing8_Tryptone
ing9_Yeast_extract["Yeast extract"]
CultureMech_001256 --> ing9_Yeast_extract
ing10_Sodium_chloride["Sodium chloride"]
CultureMech_001256 --> ing10_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing10_Sodium_chloride -.-> CHEBI_26710
ing11_Rabbit_serum["Rabbit serum"]
CultureMech_001256 --> ing11_Rabbit_serum
ing12_MEM_alpha_Modification["MEM alpha Modification"]
CultureMech_001256 --> ing12_MEM_alpha_Modification
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | HEPES | 5.1 G_PER_L |
CHEBI:46756
HEPES
|
|
| 2 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
0.5 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
4.2 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
Pyruvate
C3H3O3 87.054 g/mol
|
0.6 G_PER_L |
CHEBI:15361
pyruvate
|
|
| 5 | N-Acetylglucosamine | 0.3 G_PER_L |
CHEBI:59640
N-acetylglucosamine
|
|
| 6 |
Sodium bicarbonate
CHO3.Na 84.006 g/mol
|
1.8 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 | Yeast extract | 1.7 G_PER_L | ||
| 8 | Bacto peptone | 4.2 G_PER_L | ||
| 9 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 10 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 11 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 12 | Rabbit serum | 8.56 G_PER_L | ||
| 13 | MEM alpha Modification | 8.9 G_PER_L |
Preparation
- Combine all ingredients, adjust pH to 7.3 with 1 M NaOH and filter-sterilize
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- bskii_medium_borrelia (CultureMech:001256)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.502842Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1829 |
| 2026-01-28T19:48:35.568733Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:02.597344Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001256 |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.439330+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BSKII medium (Borrelia)" to "bskii_medium_borrelia" for consistent matching |
| 2026-06-05T07:40:51.680912+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:26.064159+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 157a5167c9b8b7aa5fd3594b8d8ff02361bcadfc5b3d90c4dcdb1b472c99bf07 |