bskii_medium_borrelia (CultureMech:001256)

Overview

External ontology

mediadive.medium:1829 (source link unavailable) — BSKII medium (Borrelia)

Applications

Microbial cultivation

pH

7.3

Notes

Source: DSMZ Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The false LB constituent expansion is withdrawn in favor of this source's bovine serum albumin input. Existing unrelated chemical annotations are preserved, not recertified. This repair does not assert equivalence with other BSK formulations or add organism-growth evidence.

Composition

flowchart LR
    CultureMech_001256["bskii_medium_borrelia"]
    ing0_HEPES["HEPES"]
    CultureMech_001256 --> ing0_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing0_HEPES -.-> CHEBI_46756
    ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
    CultureMech_001256 --> ing1_Trisodium_citrate_x_2_H2O
    CHEBI_53258["CHEBI:53258"]
    ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
    ing2_Glucose["Glucose"]
    CultureMech_001256 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Pyruvate["Pyruvate"]
    CultureMech_001256 --> ing3_Pyruvate
    CHEBI_15361["CHEBI:15361"]
    ing3_Pyruvate -.-> CHEBI_15361
    ing4_N_Acetylglucosamine["N-Acetylglucosamine"]
    CultureMech_001256 --> ing4_N_Acetylglucosamine
    CHEBI_59640["CHEBI:59640"]
    ing4_N_Acetylglucosamine -.-> CHEBI_59640
    ing5_Sodium_bicarbonate["Sodium bicarbonate"]
    CultureMech_001256 --> ing5_Sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing5_Sodium_bicarbonate -.-> CHEBI_32139
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001256 --> ing6_Yeast_extract
    ing7_Bacto_peptone["Bacto peptone"]
    CultureMech_001256 --> ing7_Bacto_peptone
    ing8_Bovine_serum_albumin["Bovine serum albumin"]
    CultureMech_001256 --> ing8_Bovine_serum_albumin
    ing9_Rabbit_serum["Rabbit serum"]
    CultureMech_001256 --> ing9_Rabbit_serum
    ing10_MEM_alpha_Modification["MEM alpha Modification"]
    CultureMech_001256 --> ing10_MEM_alpha_Modification
    ing11_Deionized_water["Deionized water"]
    CultureMech_001256 --> ing11_Deionized_water

Ingredients (12)

#	Ingredient	Concentration	Ontology	Notes
1	HEPES	5.1 G_PER_L	CHEBI:46756 HEPES
2	Trisodium citrate x 2 H2O C6H5O7.3Na 258.069 g/mol	0.5 G_PER_L	CHEBI:53258 sodium citrate
3	Glucose C6H12O6 180.156 g/mol	4.2 G_PER_L	CHEBI:17234 glucose
4	Pyruvate C3H3O3 87.054 g/mol	0.6 G_PER_L	CHEBI:15361 pyruvate
5	N-Acetylglucosamine	0.3 G_PER_L	CHEBI:59640 N-acetylglucosamine
6	Sodium bicarbonate CHO3.Na 84.006 g/mol	1.8 G_PER_L	CHEBI:32139 sodium hydrogencarbonate
7	Yeast extract	1.7 G_PER_L
8	Bacto peptone	4.2 G_PER_L		Original source component specification: Becton Dickinson.
9	Bovine serum albumin	8.5 G_PER_L		Original source component specification: Fraction V.
10	Rabbit serum	85.6 ML_PER_L
11	MEM alpha Modification	8.9 G_PER_L		Original source component specification: BioWest Cat No. P0440.
12	Deionized water			Deionized water: make up the complete medium to a final volume of 100 ml; this is not a fixed 100 ml water addition.

Preparation

Combine the recipe ingredients. The source specifies a final medium volume of 100 ml, using deionized water as makeup; no separate makeup-versus-titration order is asserted.
Adjust to pH 7.3 with 1 M NaOH.
Filter-sterilize; the source specifies no filter pore size.

Solutions

NaOH solution (1 M)

Stock composition
Ingredient	Concentration	Notes
NaOH	1 MOLAR

Titrate to final pH 7.3. The 1 M value is titrant stock strength; no fixed dose or final-medium NaOH concentration is specified.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:15.502842Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1829
2026-01-28T19:48:35.568733Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:02.597344Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001256
2026-03-17T06:30:41.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.439330+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "BSKII medium (Borrelia)" to "bskii_medium_borrelia" for consistent matching
2026-06-05T07:40:51.680912+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-08T23:24:53.639909+00:00	codex	RESTORED_SOURCE_SPECIFIC_BSK_FORMULATIONS_WITH_INGREDIENT_EVIDENCE	Restore source-specific albumin, quantity scopes, missing inputs and preparation boundaries. Preserve ID, prior history and unrelated groundings. Unknown doses, percentage bases and final volumes stay unknown; distinct formulations are not blended.