pectin_medium

CultureMech:001273 bacterial COMPLEX LIQUID
Original name: Pectin medium

Overview

External ontology
mediadive.medium:1855 (source link unavailable) — Pectin medium
Applications
  • Microbial cultivation
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001273["pectin_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001273 --> ing0_Yeast_extract
    ing1_Hipolypepton["Hipolypepton"]
    CultureMech_001273 --> ing1_Hipolypepton
    ing2_Pectin["Pectin"]
    CultureMech_001273 --> ing2_Pectin
    CHEBI_17309["CHEBI:17309"]
    ing2_Pectin -.-> CHEBI_17309
    ing3_NaCl["NaCl"]
    CultureMech_001273 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001273 --> ing4_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing5_Resazurin["Resazurin"]
    CultureMech_001273 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Yeast extract 5 G_PER_L
2 Hipolypepton 3 G_PER_L
3 Pectin 3 G_PER_L CHEBI:17309
pectin
4 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
5 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
6 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin

Preparation

  1. Add cysteine solution after the medium has been boiled and cooled under CO2. Adjust pH to 6.5 using NaOH, Distribute under N2 and autoclave.
  2. When preparing the plate medium, add 15g/L agar
  3. Autoclave at 121 °C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.552333Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1855
2026-03-13T06:19:02.729950Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001273
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.580633+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Pectin medium" to "pectin_medium" for consistent matching
2026-06-05T07:41:41.101896+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:52.428040+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint d8bc472f1c7ba0ea2144604a181b213e62a15349881a98c19b210e29c1c5c58c