Overview
- External ontology
- mediadive.medium:1855 (source link unavailable) — Pectin medium
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ
Composition
flowchart LR
CultureMech_001273["pectin_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001273 --> ing0_Yeast_extract
ing1_Hipolypepton["Hipolypepton"]
CultureMech_001273 --> ing1_Hipolypepton
ing2_Pectin["Pectin"]
CultureMech_001273 --> ing2_Pectin
CHEBI_17309["CHEBI:17309"]
ing2_Pectin -.-> CHEBI_17309
ing3_NaCl["NaCl"]
CultureMech_001273 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001273 --> ing4_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_Resazurin["Resazurin"]
CultureMech_001273 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 5 G_PER_L | ||
| 2 | Hipolypepton | 3 G_PER_L | ||
| 3 | Pectin | 3 G_PER_L |
CHEBI:17309
pectin
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
Preparation
- Add cysteine solution after the medium has been boiled and cooled under CO2. Adjust pH to 6.5 using NaOH, Distribute under N2 and autoclave.
- When preparing the plate medium, add 15g/L agar
- Autoclave at 121 °C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.552333Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1855 |
| 2026-03-13T06:19:02.729950Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001273 |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.580633+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pectin medium" to "pectin_medium" for consistent matching |
| 2026-06-05T07:41:41.101896+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m3283_score15.py | LINKED_TOGO_M3283_DERIVED_FROM | Linked TOGO M3283 as a derived variant of DSMZ Medium 1855. |