Overview
- External ontology
- mediadive.medium:1875 (source link unavailable) — Medium MYGS
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ
Composition
flowchart LR
CultureMech_001278["medium_mygs"]
ing0_MES__2__N_morpholino__ethane_sulfonic_acid_["MES [2-(N-morpholino) ethane sulfonic acid]"]
CultureMech_001278 --> ing0_MES__2__N_morpholino__ethane_sulfonic_acid_
CHEBI_39005["CHEBI:39005"]
ing0_MES__2__N_morpholino__ethane_sulfonic_acid_ -.-> CHEBI_39005
ing1_Yeast_extract["Yeast extract"]
CultureMech_001278 --> ing1_Yeast_extract
ing2_Starch["Starch"]
CultureMech_001278 --> ing2_Starch
CHEBI_28017["CHEBI:28017"]
ing2_Starch -.-> CHEBI_28017
ing3_D____Glucose["D(+)-Glucose"]
CultureMech_001278 --> ing3_D____Glucose
CHEBI_17634["CHEBI:17634"]
ing3_D____Glucose -.-> CHEBI_17634
ing4_Glycerol["Glycerol"]
CultureMech_001278 --> ing4_Glycerol
CHEBI_17754["CHEBI:17754"]
ing4_Glycerol -.-> CHEBI_17754
ing5_Agar["Agar"]
CultureMech_001278 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_HCl["HCl"]
CultureMech_001278 --> ing6_HCl
CHEBI_17883["CHEBI:17883"]
ing6_HCl -.-> CHEBI_17883
ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001278 --> ing7_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
ing8_ZnCl2["ZnCl2"]
CultureMech_001278 --> ing8_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing8_ZnCl2 -.-> CHEBI_49976
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001278 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_H3BO3["H3BO3"]
CultureMech_001278 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001278 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001278 --> ing12_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001278 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001278 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_NaOH["NaOH"]
CultureMech_001278 --> ing15_NaOH
CHEBI_32145["CHEBI:32145"]
ing15_NaOH -.-> CHEBI_32145
ing16_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001278 --> ing16_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing16_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001278 --> ing17_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001278 --> ing18_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
ing19_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001278 --> ing19_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing19_CaCl2_x_2_H2O -.-> CHEBI_86158
ing20__NH4_2HPO4["(NH4)2HPO4"]
CultureMech_001278 --> ing20__NH4_2HPO4
CHEBI_63051["CHEBI:63051"]
ing20__NH4_2HPO4 -.-> CHEBI_63051
ing21_Vitamin_B12["Vitamin B12"]
CultureMech_001278 --> ing21_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing21_Vitamin_B12 -.-> CHEBI_176843
ing22_4_aminobenzoate["4-aminobenzoate"]
CultureMech_001278 --> ing22_4_aminobenzoate
CHEBI_17836["CHEBI:17836"]
ing22_4_aminobenzoate -.-> CHEBI_17836
ing23_Biotin["Biotin"]
CultureMech_001278 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_001278 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_Hemicalcium_D_____pantothenate["Hemicalcium D-(+)-pantothenate"]
CultureMech_001278 --> ing25_Hemicalcium_D_____pantothenate
ing26_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_001278 --> ing26_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing26_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing27_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001278 --> ing27_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing27_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing28_DL_6_8_thioctic_acid["DL-6,8-thioctic acid"]
CultureMech_001278 --> ing28_DL_6_8_thioctic_acid
CHEBI_16494["CHEBI:16494"]
ing28_DL_6_8_thioctic_acid -.-> CHEBI_16494
ing29_Riboflavin["Riboflavin"]
CultureMech_001278 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_001278 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MES [2-(N-morpholino) ethane sulfonic acid]
C6H13NO4S 195.24 g/mol
|
1.95 G_PER_L |
CHEBI:39005
2-(N-morpholino)ethanesulfonic acid
|
|
| 2 | Yeast extract | 0.5 G_PER_L | ||
| 3 | Starch | 0.4 G_PER_L |
CHEBI:28017
starch
|
|
| 4 |
D(+)-Glucose
C6H12O6 180.156 g/mol
|
0.45 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 5 |
Glycerol
C3H8O3 92.094 g/mol
|
0.7 G_PER_L |
CHEBI:17754
glycerol
|
|
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 7 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 8 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 9 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 17 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 18 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 19 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
4.9 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 20 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
4.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 21 |
(NH4)2HPO4
2H4N.HO4P 132.056 g/mol
|
2.6 G_PER_L |
CHEBI:63051
diammonium hydrogen phosphate
|
|
| 22 | Vitamin B12 | 0.017 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 23 |
4-aminobenzoate
C7H6NO2 136.13 g/mol
|
0.013 G_PER_L |
CHEBI:17836
4-aminobenzoate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.003 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.033 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 26 | Hemicalcium D-(+)-pantothenate | 0.017 G_PER_L | ||
| 27 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.05 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 28 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.033 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 29 |
DL-6,8-thioctic acid
C8H14O2S2 206.332 g/mol
|
0.01 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 31 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.004 G_PER_L |
CHEBI:27470
folic acid
|
Preparation
- Adjust pH to 7.2.
- Agar und die restlichen Bestandteile getrennt autoklavieren (20 g Agar in 500 ml dest. H2O + Rest der Zutaten in 440 ml dest. H2O)
- Vitaminlösung erst nach dem Autoklavieren dazugeben
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
- Filter sterilize the solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_mygs (CultureMech:001278)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.567465Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1875 |
| 2026-01-28T19:48:18.899192Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:02.775630Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001278 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.629400+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium MYGS" to "medium_mygs" for consistent matching |
| 2026-06-05T07:41:30.220593+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.430666+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:06.483755+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:44.265074+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3737022d70b53d88f47f4cf5da98f327d275d34980379c26e2ed35d504cc7089 |