medium_mygs

CultureMech:001278 bacterial COMPLEX SOLID_AGAR
Original name: Medium MYGS

Overview

External ontology
mediadive.medium:1875 (source link unavailable) — Medium MYGS
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_001278["medium_mygs"]
    ing0_MES__2__N_morpholino__ethane_sulfonic_acid_["MES [2-(N-morpholino) ethane sulfonic acid]"]
    CultureMech_001278 --> ing0_MES__2__N_morpholino__ethane_sulfonic_acid_
    CHEBI_39005["CHEBI:39005"]
    ing0_MES__2__N_morpholino__ethane_sulfonic_acid_ -.-> CHEBI_39005
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001278 --> ing1_Yeast_extract
    ing2_Starch["Starch"]
    CultureMech_001278 --> ing2_Starch
    CHEBI_28017["CHEBI:28017"]
    ing2_Starch -.-> CHEBI_28017
    ing3_D____Glucose["D(+)-Glucose"]
    CultureMech_001278 --> ing3_D____Glucose
    CHEBI_17634["CHEBI:17634"]
    ing3_D____Glucose -.-> CHEBI_17634
    ing4_Glycerol["Glycerol"]
    CultureMech_001278 --> ing4_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing4_Glycerol -.-> CHEBI_17754
    ing5_Agar["Agar"]
    CultureMech_001278 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_HCl["HCl"]
    CultureMech_001278 --> ing6_HCl
    CHEBI_17883["CHEBI:17883"]
    ing6_HCl -.-> CHEBI_17883
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001278 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_ZnCl2["ZnCl2"]
    CultureMech_001278 --> ing8_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing8_ZnCl2 -.-> CHEBI_49976
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001278 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_H3BO3["H3BO3"]
    CultureMech_001278 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001278 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001278 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001278 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001278 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_NaOH["NaOH"]
    CultureMech_001278 --> ing15_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing15_NaOH -.-> CHEBI_32145
    ing16_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001278 --> ing16_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing16_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001278 --> ing17_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001278 --> ing18_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing19_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001278 --> ing19_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing19_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing20__NH4_2HPO4["(NH4)2HPO4"]
    CultureMech_001278 --> ing20__NH4_2HPO4
    CHEBI_63051["CHEBI:63051"]
    ing20__NH4_2HPO4 -.-> CHEBI_63051
    ing21_Vitamin_B12["Vitamin B12"]
    CultureMech_001278 --> ing21_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing21_Vitamin_B12 -.-> CHEBI_176843
    ing22_4_aminobenzoate["4-aminobenzoate"]
    CultureMech_001278 --> ing22_4_aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing22_4_aminobenzoate -.-> CHEBI_17836
    ing23_Biotin["Biotin"]
    CultureMech_001278 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001278 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_Hemicalcium_D_____pantothenate["Hemicalcium D-(+)-pantothenate"]
    CultureMech_001278 --> ing25_Hemicalcium_D_____pantothenate
    ing26_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_001278 --> ing26_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing26_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing27_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001278 --> ing27_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing27_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing28_DL_6_8_thioctic_acid["DL-6,8-thioctic acid"]
    CultureMech_001278 --> ing28_DL_6_8_thioctic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_DL_6_8_thioctic_acid -.-> CHEBI_16494
    ing29_Riboflavin["Riboflavin"]
    CultureMech_001278 --> ing29_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing29_Riboflavin -.-> CHEBI_17015
    CultureMech_001278 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 MES [2-(N-morpholino) ethane sulfonic acid]
C6H13NO4S 195.24 g/mol
1.95 G_PER_L CHEBI:39005
2-(N-morpholino)ethanesulfonic acid
2 Yeast extract 0.5 G_PER_L
3 Starch 0.4 G_PER_L CHEBI:28017
starch
4 D(+)-Glucose
C6H12O6 180.156 g/mol
0.45 G_PER_L CHEBI:17634
D-glucose
5 Glycerol
C3H8O3 92.094 g/mol
0.7 G_PER_L CHEBI:17754
glycerol
6 Agar 20 G_PER_L CHEBI:2509
agar
7 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
8 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
9 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
17 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
18 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
19 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
4.9 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
20 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
4.4 G_PER_L CHEBI:86158
calcium chloride dihydrate
21 (NH4)2HPO4
2H4N.HO4P 132.056 g/mol
2.6 G_PER_L CHEBI:63051
diammonium hydrogen phosphate
22 Vitamin B12 0.017 G_PER_L CHEBI:176843
vitamin B12
23 4-aminobenzoate
C7H6NO2 136.13 g/mol
0.013 G_PER_L CHEBI:17836
4-aminobenzoate
24 Biotin
C10H16N2O3S 244.316 g/mol
0.003 G_PER_L CHEBI:15956
biotin
25 Nicotinic acid
C6H5NO2 123.111 g/mol
0.033 G_PER_L CHEBI:15940
nicotinic acid
26 Hemicalcium D-(+)-pantothenate 0.017 G_PER_L
27 Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
0.05 G_PER_L CHEBI:131531
pyridoxamine hydrochloride
28 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.033 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
29 DL-6,8-thioctic acid
C8H14O2S2 206.332 g/mol
0.01 G_PER_L CHEBI:16494
lipoic acid
30 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
31 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid

Preparation

  1. Adjust pH to 7.2.
  2. Agar und die restlichen Bestandteile getrennt autoklavieren (20 g Agar in 500 ml dest. H2O + Rest der Zutaten in 440 ml dest. H2O)
  3. Vitaminlösung erst nach dem Autoklavieren dazugeben
  4. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
  5. Filter sterilize the solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.567465Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1875
2026-01-28T19:48:18.899192Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:02.775630Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001278
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.629400+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Medium MYGS" to "medium_mygs" for consistent matching
2026-06-05T07:41:30.220593+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.430666+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:06.483755+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.