Overview
- External ontology
- mediadive.medium:195d (source link unavailable) — KNH MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.1, 'max': 7.4}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195d.pdf
Composition
flowchart LR
CultureMech_001294["knh_medium"]
ing0_Na2SO4["Na2SO4"]
CultureMech_001294 --> ing0_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing0_Na2SO4 -.-> CHEBI_32149
ing1_KH2PO4["KH2PO4"]
CultureMech_001294 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_001294 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_001294 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001294 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_001294 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001294 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Yeast_extract["Yeast extract"]
CultureMech_001294 --> ing7_Yeast_extract
ing8_Ascorbic_acid["Ascorbic acid"]
CultureMech_001294 --> ing8_Ascorbic_acid
CHEBI_22652["CHEBI:22652"]
ing8_Ascorbic_acid -.-> CHEBI_22652
ing9_Sodium_resazurin["Sodium resazurin"]
CultureMech_001294 --> ing9_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Sodium_resazurin -.-> CHEBI_8806
ing10_Na2CO3["Na2CO3"]
CultureMech_001294 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_Na_acetate["Na-acetate"]
CultureMech_001294 --> ing11_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing11_Na_acetate -.-> CHEBI_32954
ing12_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_001294 --> ing12_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing12_Tris_HCl_buffer -.-> CHEBI_9754
ing13_Na3_NTA_x_H2O["Na3-NTA x H2O"]
CultureMech_001294 --> ing13_Na3_NTA_x_H2O
ing14_TiCl3["TiCl3"]
CultureMech_001294 --> ing14_TiCl3
ing15_NaOH["NaOH"]
CultureMech_001294 --> ing15_NaOH
CHEBI_32145["CHEBI:32145"]
ing15_NaOH -.-> CHEBI_32145
ing16_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001294 --> ing16_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing16_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001294 --> ing17_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing18_HCl["HCl"]
CultureMech_001294 --> ing18_HCl
CHEBI_17883["CHEBI:17883"]
ing18_HCl -.-> CHEBI_17883
ing19_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001294 --> ing19_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing19_FeCl2_x_4_H2O -.-> CHEBI_86249
ing20_ZnCl2["ZnCl2"]
CultureMech_001294 --> ing20_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing20_ZnCl2 -.-> CHEBI_49976
ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001294 --> ing21_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
ing22_H3BO3["H3BO3"]
CultureMech_001294 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001294 --> ing23_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
ing24_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001294 --> ing24_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing24_CuCl2_x_2_H2O -.-> CHEBI_86318
ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001294 --> ing25_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001294 --> ing26_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing27_Biotin["Biotin"]
CultureMech_001294 --> ing27_Biotin
CHEBI_15956["CHEBI:15956"]
ing27_Biotin -.-> CHEBI_15956
ing28_Folic_acid["Folic acid"]
CultureMech_001294 --> ing28_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing28_Folic_acid -.-> CHEBI_27470
ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001294 --> ing29_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
CultureMech_001294 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.15126 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.210084 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.315126 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
22.0588 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.15126 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.52521 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.157563 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 | Yeast extract | 0.52521 G_PER_L | ||
| 9 | Ascorbic acid | 0.105042 G_PER_L |
CHEBI:22652
ascorbic acid
|
|
| 10 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00052521 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
50 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
70 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 13 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
7.2 G_PER_L |
CHEBI:9754
tris
|
|
| 14 | Na3-NTA x H2O | 4.8 G_PER_L | ||
| 15 | TiCl3 | 6.34615 G_PER_L | ||
| 16 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 17 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 18 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 19 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 20 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 21 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 22 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 24 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 25 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 26 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 27 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 28 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 29 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 30 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 31 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 32 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 33 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 34 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 35 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 36 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 37 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Solution A is sparged with 80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere in anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is autoclaved under 100% N2 gas. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium solutions B to E are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4.
- After inoculation add 1 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.
- Sterilize by filtration.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- knh_medium (CultureMech:001294)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.470798Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 195d |
| 2026-02-06T05:10:28.800980Z | invalid-chebi-removal | Removed 2 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:02.952109Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001294 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.820146+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "KNH MEDIUM" to "knh_medium" for consistent matching |
| 2026-05-20T06:01:23.874692+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.1-7.4' -> {'min': 7.1, 'max': 7.4} |
| 2026-06-05T07:41:10.441754+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.381602+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.656916+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint db5b17eb8ee72e95349f2c1910a166c3424f18b0b8f11986f3b7acadb01c7f0e |