knh_medium

CultureMech:001294 bacterial COMPLEX LIQUID
Original name: KNH MEDIUM

Overview

External ontology
mediadive.medium:195d (source link unavailable) — KNH MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.1, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195d.pdf

Composition

flowchart LR
    CultureMech_001294["knh_medium"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_001294 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001294 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001294 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_001294 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001294 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_001294 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001294 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_001294 --> ing7_Yeast_extract
    ing8_Ascorbic_acid["Ascorbic acid"]
    CultureMech_001294 --> ing8_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing8_Ascorbic_acid -.-> CHEBI_22652
    ing9_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001294 --> ing9_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Sodium_resazurin -.-> CHEBI_8806
    ing10_Na2CO3["Na2CO3"]
    CultureMech_001294 --> ing10_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing10_Na2CO3 -.-> CHEBI_29377
    ing11_Na_acetate["Na-acetate"]
    CultureMech_001294 --> ing11_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing11_Na_acetate -.-> CHEBI_32954
    ing12_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_001294 --> ing12_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing12_Tris_HCl_buffer -.-> CHEBI_9754
    ing13_Na3_NTA_x_H2O["Na3-NTA x H2O"]
    CultureMech_001294 --> ing13_Na3_NTA_x_H2O
    ing14_TiCl3["TiCl3"]
    CultureMech_001294 --> ing14_TiCl3
    ing15_NaOH["NaOH"]
    CultureMech_001294 --> ing15_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing15_NaOH -.-> CHEBI_32145
    ing16_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001294 --> ing16_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing16_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001294 --> ing17_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing18_HCl["HCl"]
    CultureMech_001294 --> ing18_HCl
    CHEBI_17883["CHEBI:17883"]
    ing18_HCl -.-> CHEBI_17883
    ing19_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001294 --> ing19_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing19_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing20_ZnCl2["ZnCl2"]
    CultureMech_001294 --> ing20_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing20_ZnCl2 -.-> CHEBI_49976
    ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001294 --> ing21_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing22_H3BO3["H3BO3"]
    CultureMech_001294 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001294 --> ing23_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing24_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001294 --> ing24_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing24_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001294 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001294 --> ing26_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing27_Biotin["Biotin"]
    CultureMech_001294 --> ing27_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing27_Biotin -.-> CHEBI_15956
    ing28_Folic_acid["Folic acid"]
    CultureMech_001294 --> ing28_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing28_Folic_acid -.-> CHEBI_27470
    ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001294 --> ing29_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
    CultureMech_001294 --> more["...7 more"]

Ingredients (37)

# Ingredient Concentration Ontology Notes
1 Na2SO4
2Na.O4S 142.043 g/mol
3.15126 G_PER_L CHEBI:32149
sodium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.210084 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.315126 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
22.0588 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
3.15126 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 KCl
Cl.K 74.551 g/mol
0.52521 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.157563 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Yeast extract 0.52521 G_PER_L
9 Ascorbic acid 0.105042 G_PER_L CHEBI:22652
ascorbic acid
10 Sodium resazurin
C12H7NO4 229.191 g/mol
0.00052521 G_PER_L CHEBI:8806
Resazurin
11 Na2CO3
CO3.2Na 105.988 g/mol
50 G_PER_L CHEBI:29377
sodium carbonate
12 Na-acetate
C2H3O2.Na 82.034 g/mol
70 G_PER_L CHEBI:32954
sodium acetate
13 Tris-HCl buffer
C4H11NO3 121.136 g/mol
7.2 G_PER_L CHEBI:9754
tris
14 Na3-NTA x H2O 4.8 G_PER_L
15 TiCl3 6.34615 G_PER_L
16 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
17 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
18 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
19 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
20 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
21 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
22 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
23 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
24 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
25 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
26 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
27 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
28 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
29 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
30 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
31 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
32 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
33 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
34 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
35 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
36 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
37 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Solution A is sparged with 80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere in anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is autoclaved under 100% N2 gas. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium solutions B to E are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4.
  2. After inoculation add 1 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.
  3. Sterilize by filtration.
  4. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.470798Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 195d
2026-02-06T05:10:28.800980Z invalid-chebi-removal Removed 2 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:02.952109Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001294
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.820146+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "KNH MEDIUM" to "knh_medium" for consistent matching
2026-05-20T06:01:23.874692+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.1-7.4' -> {'min': 7.1, 'max': 7.4}
2026-06-05T07:41:10.441754+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.381602+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.