Overview
- External ontology
- mediadive.medium:1a (source link unavailable) — REACTIVATION WITH LIQUID MEDIUM 1
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'nutrient_agar', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1', 'original_category': 'bacterial'}
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1a.pdf
Composition
flowchart LR
CultureMech_001298["reactivation_with_liquid_medium_1"]
ing0_Peptone["Peptone"]
CultureMech_001298 --> ing0_Peptone
ing1_Meat_extract["Meat extract"]
CultureMech_001298 --> ing1_Meat_extract
ing2_Agar["Agar"]
CultureMech_001298 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5 G_PER_L | ||
| 2 | Meat extract | 3 G_PER_L | ||
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
- Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- nutrient_agar (CultureMech:001297)
- reactivation_with_liquid_medium_1 (CultureMech:001298)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:09.956024Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1a |
| 2026-03-13T06:19:02.987652Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001298 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:14.860098+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "REACTIVATION WITH LIQUID MEDIUM 1" to "reactivation_with_liquid_medium_1" for consistent matching |
| 2026-06-05T07:41:44.068265+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:51.656707+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: nutrient_agar, reactivation_with_liquid_medium_1 |
| 2026-08-06T03:05:51.656748+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 7d42c4b3d2a02cfea7071bab1c6d93b36cec184b9018ec00a03737e6e8067149 |