nutrient_agar

CultureMech:001297 bacterial COMPLEX SOLID_AGAR
Original name: NUTRIENT AGAR

Overview

External ontology
mediadive.medium:1 (source link unavailable) — NUTRIENT AGAR
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1.pdf Cross-reference: ATCC:1 (NUTRIENT AGAR)

Composition

flowchart LR
    CultureMech_001297["nutrient_agar"]
    ing0_Peptone["Peptone"]
    CultureMech_001297 --> ing0_Peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_001297 --> ing1_Meat_extract
    ing2_Agar["Agar"]
    CultureMech_001297 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Peptone 5 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
2 Meat extract 3 G_PER_L
3 Agar 15 G_PER_L CHEBI:2509
agar
(for solid medium)

Preparation

  1. Adjust pH to 7.0.
  2. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:09.954458Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1
2026-02-20T07:33:34.000000Z atcc-crossref-backfill Added ATCC cross-reference ATCC:1 verified equivalent (verified 2026-02-19)
2026-03-13T06:19:02.984675Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001297
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.856665+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NUTRIENT AGAR" to "nutrient_agar" for consistent matching
2026-06-05T07:41:40.108258+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m2339_m2340_score15.py LINKED_TOGO_M2339_M2340_DSMZ_MEDIUM_1 Linked TOGO M2339 as a source duplicate and TOGO M2340 as a 10 mg/L MnSO4 x H2O supplemented variant of DSMZ Medium 1.
2026-09-11T00:00:00-07:00 repair_togo_m3116_score15.py LINKED_TOGO_M3116_SOURCE_DUPLICATE Linked TOGO M3116 as a source duplicate of DSMZ Medium 1.
2026-09-12T00:00:00-07:00 repair_togo_m2200_horse_serum_score15.py LINKED_TOGO_M2200_HORSE_SERUM_VARIANT Linked TOGO M2200 as a 20% horse-serum variant of DSMZ Medium 1.
2026-09-13T00:00:00-07:00 repair_bacterial_score10_exact_terms_batch6.py GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH6 Applied local mappings for Peptone.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.