natronobacterium_medium

CultureMech:001304 archaea COMPLEX SOLID_AGAR
Original name: NATRONOBACTERIUM MEDIUM

Overview

External ontology
mediadive.medium:205 (source link unavailable) — NATRONOBACTERIUM MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'jcm_medium_no_166', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J166', 'original_category': 'bacterial'}
pH
8.5
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium205.pdf

Composition

flowchart LR
    CultureMech_001304["natronobacterium_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_001304 --> ing0_Casamino_acids
    ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
    CultureMech_001304 --> ing1_Trisodium_citrate_x_2_H2O
    CHEBI_53258["CHEBI:53258"]
    ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
    ing2_Glutamic_acid["Glutamic acid"]
    CultureMech_001304 --> ing2_Glutamic_acid
    CHEBI_18237["CHEBI:18237"]
    ing2_Glutamic_acid -.-> CHEBI_18237
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001304 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_KCl["KCl"]
    CultureMech_001304 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaCl["NaCl"]
    CultureMech_001304 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Agar["Agar"]
    CultureMech_001304 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Casamino acids 15 G_PER_L
2 Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
3 Glutamic acid
C5H9NO4 147.13 g/mol
2.5 G_PER_L CHEBI:18237
glutamic acid
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
6 NaCl
Cl.Na 58.443 g/mol
250 G_PER_L CHEBI:26710
sodium chloride
7 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Dissolve agar by heating before adding sodium chloride. Adjust pH to 7.0 before autoclaving. Adjust pH to 8.5 with sterile 5% Na2CO3 after heat sterilization of the medium.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:10.499179Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 205
2026-01-28T19:48:43.287261Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:03.047916Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001304
2026-03-17T06:30:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.930105+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NATRONOBACTERIUM MEDIUM" to "natronobacterium_medium" for consistent matching
2026-06-05T07:41:39.379208+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.362040+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-08-06T03:04:32.070527+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: natronobacterium_medium, jcm_medium_no_166
2026-08-06T03:04:32.070571+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint c33ad55b436bd5841880ac0ce9e503498a86e166b87033c86d7daa385e8020d6