Overview
- External ontology
- mediadive.medium:J166 (source link unavailable) — JCM MEDIUM No. 166
- Applications
-
- Microbial cultivation
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=166
Composition
flowchart LR
CultureMech_002525["jcm_medium_no_166"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_002525 --> ing0_Casamino_acids
ing1_Trisodium_citrate["Trisodium citrate"]
CultureMech_002525 --> ing1_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing1_Trisodium_citrate -.-> CHEBI_53258
ing2_Glutamic_acid["Glutamic acid"]
CultureMech_002525 --> ing2_Glutamic_acid
CHEBI_18237["CHEBI:18237"]
ing2_Glutamic_acid -.-> CHEBI_18237
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002525 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_KCl["KCl"]
CultureMech_002525 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaCl["NaCl"]
CultureMech_002525 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Agar["Agar"]
CultureMech_002525 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 15 G_PER_L | ||
| 2 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 3 |
Glutamic acid
C5H9NO4 147.13 g/mol
|
2.5 G_PER_L |
CHEBI:18237
glutamic acid
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
250 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0. After autoclaving, readjust pH to 8.5 with a sterile 5% Na2CO3 solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.860866Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J166 |
| 2026-01-28T19:48:28.147815Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:13.961945Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002525 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.144162+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 166" to "jcm_medium_no_166" for consistent matching |
| 2026-06-05T07:41:09.826142+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:40.177817+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |