Overview
- External ontology
- mediadive.medium:215c (source link unavailable) — BHI MEDIUM FOR STRICT ANAEROBES
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.6}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215c.pdf Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001317["bhi_medium_for_strict_anaerobes"]
ing0_Calf_brains["Calf brains"]
CultureMech_001317 --> ing0_Calf_brains
ing1_Beef_heart["Beef heart"]
CultureMech_001317 --> ing1_Beef_heart
ing2_Proteose_peptone["Proteose peptone"]
CultureMech_001317 --> ing2_Proteose_peptone
ing3_Dextrose["Dextrose"]
CultureMech_001317 --> ing3_Dextrose
CHEBI_17634["CHEBI:17634"]
ing3_Dextrose -.-> CHEBI_17634
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_001317 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Disodium_phosphate["Disodium phosphate"]
CultureMech_001317 --> ing5_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing5_Disodium_phosphate -.-> CHEBI_34683
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001317 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001317 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 2 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 3 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 4 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 6 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.25 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.25 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- bhi_medium_for_strict_anaerobes (CultureMech:001317)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.538628Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 215c |
| 2026-03-13T06:19:03.178379Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001317 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.072507+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BHI MEDIUM FOR STRICT ANAEROBES" to "bhi_medium_for_strict_anaerobes" for consistent matching |
| 2026-05-20T06:01:09.871727+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.6' -> {'min': 7.2, 'max': 7.6} |
| 2026-06-05T07:40:50.902142+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:25.143757+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bc50d0b7f292df0e8d533ffc2710cdb64c4913457a0a9d8a8e5c040c1ce11c4c |