bhi_medium_for_strict_anaerobes

CultureMech:001317 bacterial COMPLEX LIQUID
Original name: BHI MEDIUM FOR STRICT ANAEROBES

Overview

External ontology
mediadive.medium:215c (source link unavailable) — BHI MEDIUM FOR STRICT ANAEROBES
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.6}
Notes
MediaDive and DSMZ Medium 215c list BHI Medium for Strict Anaerobes as 37 g/L Brain heart infusion, 0.25 g/L L-Cysteine HCl x H2O, 0.25 g/L Na2S x 9 H2O, and distilled water, prepared anoxically under 100% N2 with the reducing agents added from sterile anoxic solutions after autoclaving.

Composition

flowchart LR
    CultureMech_001317["bhi_medium_for_strict_anaerobes"]
    ing0_Brain_heart_infusion["Brain heart infusion"]
    CultureMech_001317 --> ing0_Brain_heart_infusion
    ing1_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001317 --> ing1_L_Cysteine_HCl_x_H2O
    ing2_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001317 --> ing2_Na2S_x_9_H2O
    ing3_Distilled_water["Distilled water"]
    CultureMech_001317 --> ing3_Distilled_water

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Brain heart infusion 37 G_PER_L mediadive.compound:186 (source link unavailable)
Brain heart infusion
 DSMZ Medium 215c lists Brain heart infusion at 37 g/L as an opaque rich-meat product.
2 L-Cysteine HCl x H2O 0.25 G_PER_L mediadive.compound:770 (source link unavailable)
L-Cysteine HCl x H2O
 DSMZ Medium 215c lists 0.25 g/L L-Cysteine HCl x H2O.
3 Na2S x 9 H2O 0.25 G_PER_L mediadive.compound:54 (source link unavailable)
Na2S x 9 H2O
 DSMZ Medium 215c lists 0.25 g/L Na2S x 9 H2O.
4 Distilled water 1000 ML_PER_L mediadive.compound:4 (source link unavailable)
Distilled water
 DSMZ Medium 215c brings the recipe to one liter with distilled water.

Preparation

  1. Prepare the medium anoxically under 100% N2 gas atmosphere.
  2. Add L-Cysteine HCl x H2O and Na2S x 9 H2O after autoclaving from sterile anoxic solutions prepared under N2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.538628Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 215c
2026-03-13T06:19:03.178379Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001317
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.072507+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BHI MEDIUM FOR STRICT ANAEROBES" to "bhi_medium_for_strict_anaerobes" for consistent matching
2026-05-20T06:01:09.871727+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.6' -> {'min': 7.2, 'max': 7.6}
2026-06-05T07:40:50.902142+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_komodo_215c_strict_anaerobes_score15.py RESOLVED_DSMZ_KOMODO_215C_BHI_SCORE15 MediaDive and DSMZ Medium 215c list BHI Medium for Strict Anaerobes as 37 g/L Brain heart infusion, 0.25 g/L L-Cysteine HCl x H2O, 0.25 g/L Na2S x 9 H2O, and distilled water, prepared anoxically under 100% N2 with the reducing agents added from sterile anoxic solutions after autoclaving.