Overview
- External ontology
- mediadive.medium:241 (source link unavailable) — GLUTAMINE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium241.pdf
Composition
flowchart LR
CultureMech_001343["glutamine_medium"]
ing0_Peptone["Peptone"]
CultureMech_001343 --> ing0_Peptone
ing1_Meat_extract["Meat extract"]
CultureMech_001343 --> ing1_Meat_extract
ing2_Agar["Agar"]
CultureMech_001343 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_L_Glutamine["L-Glutamine"]
CultureMech_001343 --> ing3_L_Glutamine
CHEBI_18050["CHEBI:18050"]
ing3_L_Glutamine -.-> CHEBI_18050
ing4_Tryptophan["Tryptophan"]
CultureMech_001343 --> ing4_Tryptophan
CHEBI_27897["CHEBI:27897"]
ing4_Tryptophan -.-> CHEBI_27897
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5 G_PER_L | ||
| 2 | Meat extract | 3 G_PER_L | ||
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if necessary) |
| 4 |
L-Glutamine
C5H10N2O3 146.146 g/mol
|
0.1 G_PER_L |
CHEBI:18050
L-glutamine
|
|
| 5 |
Tryptophan
C11H12N2O2 204.229 g/mol
|
0.1 G_PER_L |
CHEBI:27897
tryptophan
|
Preparation
- Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- glutamine_medium (CultureMech:001343)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.580273Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 241 |
| 2026-03-13T06:19:03.299941Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001343 |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.209626+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GLUTAMINE MEDIUM" to "glutamine_medium" for consistent matching |
| 2026-06-05T07:41:06.534476+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:36.329900+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 051f0b7b500ebb4a5e8a83d3f6e1f5f36eb7ed4af25017921378d0418cea69a1 |