glutamine_medium

CultureMech:001343 bacterial COMPLEX SOLID_AGAR
Original name: GLUTAMINE MEDIUM

Overview

External ontology
mediadive.medium:241 (source link unavailable) — GLUTAMINE MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium241.pdf

Composition

flowchart LR
    CultureMech_001343["glutamine_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_001343 --> ing0_Peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_001343 --> ing1_Meat_extract
    ing2_Agar["Agar"]
    CultureMech_001343 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_L_Glutamine["L-Glutamine"]
    CultureMech_001343 --> ing3_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing3_L_Glutamine -.-> CHEBI_18050
    ing4_Tryptophan["Tryptophan"]
    CultureMech_001343 --> ing4_Tryptophan
    CHEBI_27897["CHEBI:27897"]
    ing4_Tryptophan -.-> CHEBI_27897

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Peptone 5 G_PER_L
2 Meat extract 3 G_PER_L
3 Agar 15 G_PER_L CHEBI:2509
agar
 (if necessary)
4 L-Glutamine
C5H10N2O3 146.146 g/mol
0.1 G_PER_L CHEBI:18050
L-glutamine
5 Tryptophan
C11H12N2O2 204.229 g/mol
0.1 G_PER_L CHEBI:27897
tryptophan

Preparation

  1. Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.580273Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 241
2026-03-13T06:19:03.299941Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001343
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.209626+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GLUTAMINE MEDIUM" to "glutamine_medium" for consistent matching
2026-06-05T07:41:06.534476+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.