Overview
- External ontology
- mediadive.medium:249 (source link unavailable) — FAECALICATENA OROTICA MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'orotic_acid_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:249', 'original_category': 'bacterial'}
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium249.pdf
Composition
flowchart LR
CultureMech_001348["faecalicatena_orotica_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_001348 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001348 --> ing1_Yeast_extract
ing2_KH2PO4["KH2PO4"]
CultureMech_001348 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4["K2HPO4"]
CultureMech_001348 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Na_orotate["Na-orotate"]
CultureMech_001348 --> ing4_Na_orotate
CHEBI_132101["CHEBI:132101"]
ing4_Na_orotate -.-> CHEBI_132101
ing5_Riboflavin["Riboflavin"]
CultureMech_001348 --> ing5_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing5_Riboflavin -.-> CHEBI_17015
ing6_Resazurin["Resazurin"]
CultureMech_001348 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Na_thioglycolate["Na-thioglycolate"]
CultureMech_001348 --> ing7_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing7_Na_thioglycolate -.-> CHEBI_86481
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 5 G_PER_L | ||
| 2 | Yeast extract | 0.5 G_PER_L | ||
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.36 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6.95 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Na-orotate
C5H3N2O4.Na 178.079 g/mol
|
2.5 G_PER_L |
CHEBI:132101
sodium orotate
|
|
| 6 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.015 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.5 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Preparation
- Dissolve ingredients and adjust pH to 7.5. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- faecalicatena_orotica_medium (CultureMech:001348)
- orotic_acid_medium (CultureMech:004621)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.591003Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 249 |
| 2026-03-13T06:19:03.325290Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001348 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.238470+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "FAECALICATENA OROTICA MEDIUM" to "faecalicatena_orotica_medium" for consistent matching |
| 2026-06-05T07:40:59.838789+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.770484+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:33.847971+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: faecalicatena_orotica_medium, orotic_acid_medium |
| 2026-08-06T03:05:33.848008+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 0eac8af5813480c576c30eae94119cc31225df501c2e63d92694d31444c78747 |