orotic_acid_medium (CultureMech:004621)

Overview

External ontology

komodo.medium:249 (source link unavailable) — OROTIC ACID medium

Applications

Microbial cultivation

pH

7.5

Notes

Source: KOMODO ModelSEED | ID: 249 | DSMZ Medium: 249 (mediadive.medium:249) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_004621["orotic_acid_medium"]
    ing0_Tryptone["Tryptone"]
    CultureMech_004621 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_004621 --> ing1_Yeast_extract
    ing2_KH2PO4["KH2PO4"]
    CultureMech_004621 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_K2HPO4["K2HPO4"]
    CultureMech_004621 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Na_orotate["Na-orotate"]
    CultureMech_004621 --> ing4_Na_orotate
    CHEBI_132101["CHEBI:132101"]
    ing4_Na_orotate -.-> CHEBI_132101
    ing5_Riboflavin["Riboflavin"]
    CultureMech_004621 --> ing5_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing5_Riboflavin -.-> CHEBI_17015
    ing6_Resazurin["Resazurin"]
    CultureMech_004621 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Na_thioglycolate["Na-thioglycolate"]
    CultureMech_004621 --> ing7_Na_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing7_Na_thioglycolate -.-> CHEBI_86481

Ingredients (8)

#	Ingredient	Concentration	Ontology
1	Tryptone	5 G_PER_L
2	Yeast extract	0.5 G_PER_L
3	KH2PO4 H2O4P.K 136.084 g/mol	1.36 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
4	K2HPO4 HO4P.2K 174.174 g/mol	6.95 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
5	Na-orotate C5H3N2O4.Na 178.079 g/mol	2.5 G_PER_L	CHEBI:132101 sodium orotate
6	Riboflavin C17H20N4O6 376.369 g/mol	0.015 G_PER_L	CHEBI:17015 riboflavin
7	Resazurin C12H7NO4 229.191 g/mol	0.001 G_PER_L	CHEBI:8806 Resazurin
8	Na-thioglycolate C2H3O2S.Na 114.101 g/mol	0.5 G_PER_L	CHEBI:86481 sodium thioglycolate

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 249
2026-02-03T11:19:19.825029	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 249
2026-03-13T06:19:27.718026Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004621
2026-03-17T06:30:57.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:42.981003+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "OROTIC ACID medium" to "orotic_acid_medium" for consistent matching
2026-06-05T07:41:40.607165+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.806044+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T22:47:39.592416+00:00	codex	CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 249 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants.